Two-Photon Tracer for Human Epidermal Growth Factor Receptor-2: Detection of Breast Cancer in a Live Tissue
| Autor: | Chang Su Lim, Dong Eun Kang, Man So Han, Kyu Cheol Paik, Seung Taek Hong, Ji-Woo Choi, Bong Rae Cho |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Absorption (pharmacology) Light Receptor ErbB-2 Recombinant Fusion Proteins Breast Neoplasms Nanotechnology Analytical Chemistry HeLa 03 medical and health sciences Breast cancer Two-photon excitation microscopy Cell Line Tumor TRACER Microscopy medicine Animals Humans Fluorescent tracer Human Epidermal Growth Factor Receptor 2 Benzofurans Fluorescent Dyes Mice Inbred BALB C biology Chemistry biology.organism_classification medicine.disease 030104 developmental biology Pyrazines Biophysics |
| Zdroj: | Analytical Chemistry. 88:9412-9418 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.6b00912 |
| Popis: | We have developed a two-photon fluorescent tracer (Pyr-affibody) that shows high selectivity for human epidermal growth factor receptor-2 (HER-2). Pyr-affibody showed absorption and emission maxima at 439 and 574 nm, respectively, with a two-photon absorption cross-section value of 40 × 10–50 cm4s/photon (GM) at 750 nm in aqueous buffer solution. The effective two-photon action cross-section value measured in HeLa cells was 600 GM at 730 nm, a value sufficient to obtain bright two-photon microscopy (TPM) images. Using Pyr-affibody, it was possible to detect HER-2 overexpressing cells and breast cancers at a depth of 90–130 μm in live mouse tissue by TPM. |
| Databáze: | OpenAIRE |
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