Ultrastructure of Bacteroides Species: Bacteroides asaccharolyticus, Bacteroides fragilis, Bacteroides melaninogenicus Subspecies melaninogenicus, and B. melaninogenicus Subspecies intermedins
| Autor: | S. C. Holt, D. D. L. Woo, E. R. Leadbetter |
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| Rok vydání: | 1979 |
| Předmět: |
Ruthenium red
Subspecies Prevotella melaninogenica Microbiology Bacteroides fragilis Fixatives chemistry.chemical_compound stomatognathic system Bacteroides Immunology and Allergy Staining and Labeling biology Thorium Osmium biology.organism_classification Ruthenium Red Staining stomatognathic diseases Infectious Diseases chemistry Glutaral Ultrastructure Bacteroides melaninogenicus |
| Zdroj: | Journal of Infectious Diseases. 139:534-546 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/139.5.534 |
| Popis: | Representative strains of two subspecies of Bacteroides melaninogenicus (subspecies melaninogenicus and subspecies intermedius) and Bacteroides asaccharolyticus as well as B. asaccharolyticus strain 536B isolated from a human perirectal abscess and Bacteroides fragilis ATCC 25285 were examined by glutaraldehyde-osmium fixation, ruthenium red fixation and staining, and thorium hydroxyde staining as well as by the physical preparative techniques of critical point drying--transmission electron microscopy (CPD--TEM) and scanning electron microscopy (SEM). All strains, with the exception of B. fragilis 25285, possessed an electron-dense material external to their outer membranes. Ruthenium red staining further revealed a layer, external to the surface of the outer membrane, that was distinct for each species examined. Thorium hydroxide, as well as CPD--TEM and SEM, showed the cells to be interconnected by thin fibers that not only connected adjacent cells but also traversed several microns to connect cell aggregates. |
| Databáze: | OpenAIRE |
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