Partial purification of human prostatic 5 alpha-reductase (3-oxo-5 alpha-steroid:NADP+ 4-ene-oxido-reductase; EC 1.3.1.22) in a stable and active form
| Autor: | N.S.E. Sargent, F.K. Habib |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Nuclear Envelope Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Detergents Prostatic Hyperplasia Reductase Biology Biochemistry Steroid chemistry.chemical_compound Endocrinology 3-Oxo-5-alpha-Steroid 4-Dehydrogenase Prostate medicine Humans Protease Inhibitors Molecular Biology Ene reaction chemistry.chemical_classification Octyl glucoside Cell Biology Lipids Molecular Weight Kinetics medicine.anatomical_structure Enzyme chemistry Ionic strength Molecular Medicine Specific activity |
| Zdroj: | The Journal of steroid biochemistry and molecular biology. 38(1) |
| ISSN: | 0960-0760 |
| Popis: | Human hyperplastic prostate tissue was homogenised in high ionic strength buffer and the post nuclear homogenate was incubated with 0.8% octyl glucoside and bovine brain lipids. Dialysis of the resulting liposome suspension yielded a preparation in which 5 alpha-reductase was active and stable for at least three weeks and showed an increase in specific activity (Vmax +/- SD = 48.9 +/- 7.4 pmol DHT/mg protein/ml) over that of the starting homogenate (Vmax +/- SD = 5.6 +/- 1.5 pmol DHT/mg protein/min) of 8.7 times. |
| Databáze: | OpenAIRE |
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