Secretory PLA2 specific single domain antibody neutralizes Russell viper venom induced cellular and organismal toxicity
| Autor: | Abhishek Dubey, Sharvan Sehrawat, Manpreet Kaur, Madhu Khatri |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Phage display
biology Chemistry Antibodies Monoclonal Viper Venoms Single-Domain Antibodies Toxicology Molecular biology In vitro Jurkat Cells Phospholipases A2 Single-domain antibody Russell viper venom In vivo Monoclonal Toxicity biology.protein Animals Humans Russell's Viper Antibody Camelids New World Zebrafish |
| Zdroj: | Toxicon. 172:15-18 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/j.toxicon.2019.10.240 |
| Popis: | Despite continued destruction of human lives by snakebites, appreciable improvements in immunotherapies have not been made. We selected and characterized venom-specific single domain antibodies (sdAbs) from a constructed phage display library of camelid variable region of heavy chain of the heavy chain antibodies (VHHs). Secretory phospholipase A2-specific sdAbs neutralized venom-induced toxicity in vitro and in vivo. Such monoclonal sdAbs could serve as an alternative to help manage snakebites to save lives. |
| Databáze: | OpenAIRE |
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