An interobserver reproducibility analysis of size-set semiautomatic counting for Ki67 assessment in breast cancer
| Autor: | Xi long Zhao, Shu ling Song, Yuan yuan Wang, Wen xing Zhao, Ju lun Yang, Lei Li, Hong Bu, Li Wang, Wen tao Yang, Yi xing Wang, Cheng gang Yang, Wen mang Xu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Wilcoxon signed-rank test
Intraclass correlation Interobserver reproducibility Breast Neoplasms Ki67 label index Assessment lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine Breast cancer Image Interpretation Computer-Assisted Humans Medicine 030212 general & internal medicine Observer Variation Reproducibility business.industry Immunochemistry Carcinoma Ductal Breast Reproducibility of Results General Medicine medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens Immunohistochemistry Predictive value Ki-67 Antigen 030220 oncology & carcinogenesis Digital image analysis Assessment methods Female Original Article Surgery business Nuclear medicine |
| Zdroj: | Breast, Vol 49, Iss, Pp 225-232 (2020) The Breast : official journal of the European Society of Mastology |
| ISSN: | 1532-3080 |
| Popis: | Purpose The proliferation marker Ki67 has prognostic and predictive values in breast cancer, and the cutoff of the Ki67 label index (LI) is a key index for chemotherapy. However, poor interobserver consistency in Ki67 assessment has limited the clinical use of Ki67, especially in luminal cancers. Here, we reported a modified Ki67 assessment method, size-set semiautomatic counting (SSSAC) and investigated its interobserver reproducibility. Methods One hundred invasive breast cancer tissues were set immunostained for Ki67 in one laboratory, scanned as digital slides, and sent to 41 pathologists at the laboratories of 16 hospitals for Ki67 LI assessment using size-set semiautomatic counting (SSSAC), size-set visual assessment (SSVA) and size-set digital image analysis (SSDIA) with a specific image viewing software (Aperio Image Scope, Leica, Germany). The intraclass correlation coefficient (ICC) and Bland-Altman plot were used to evaluate interobserver reproducibility. The Wilcoxon signed-rank test was used to analyze the difference in the Ki67 values assessed by SSSAC and SSDIA. Results SSSAC demonstrated better interobserver reproducibility (ICC = 0.942) than SSVA (ICC = 0.802). The interobserver reproducibility was better in Ki67 homogeneously stained slides and centralized hot-spot slides than in scattered hot-spot slides. The Ki67 value assessed with SSSAC was obviously higher than that assessed with SSDIA (negative ranks (SSDIA SSSAC): N = 17, sum of ranks = 478.50; Z = −6.837; P Highlights • A modified method for Ki67 LI assessment based on digital image, size-set semiautomatic counting (SSSAC). • Investigated the interobserver reproducibility of SSSAC for Ki67 LI assessment of 100 cases in 41 pathologists of 16 hospitals. • SSSAC has better interobserver reproducibility than current visual assessment and was more accurate than DIA in Ki67 assessment. • SSSAC is a better choice for Ki67 LI assessment. |
| Databáze: | OpenAIRE |
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