Characteristics of Actin Fibers and Ultrastructure of the Contact Regions Involved in the Separation of Blastomeres of Two-Cell Mouse Embryos, Frozen–Thawed without the Zona Pellucida
| Autor: | Mercè Martí, Josep Santaló, J. Egozcue, Montserrat Ponsà, Mark Grossmann |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Blastomeres Cryobiology Cell Separation In Vitro Techniques Biology General Biochemistry Genetics and Molecular Biology Cryopreservation Mice Cell Adhesion medicine Animals Zona pellucida Zona Pellucida Actin Embryo General Medicine Blastomere Anatomy Embryo Mammalian Actins Cell biology Microscopy Electron Midbody medicine.anatomical_structure Microscopy Fluorescence embryonic structures Microscopy Electron Scanning Ultrastructure Female General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 34:94-106 |
| ISSN: | 0011-2240 |
| DOI: | 10.1006/cryo.1996.1983 |
| Popis: | Freezing of embryos deprived of the zona pellucida (ZP) decreases their survival rate immediately after thawing, and gives rise to the separation of their blastomeres in a high percentage of cases. We have studied the ultrastructure and the characteristics of actin fibers in the cell-to-cell contact region in mouse embryos frozen–thawed without the ZP at the two-cell stage. Our results indicate that most of the embryos that retain their blastomeres united after freezing and thawing show either the presence of a midbody, or a contact region with a close apposition of the plasma membranes but without an organized actin cortex in their contact region. Only a small percentage of embryos that retain their blastomeres united after freezing and thawing show a contact region with widely separated plasma membranes and an organized actin cytocortex. |
| Databáze: | OpenAIRE |
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