A Heterotypic Tridimensional Model to Study the Interaction of Macrophages and Glioblastoma In Vitro

Autor: Andrea Emilse Errasti, Eugenio Antonio Carrera Silva, Ivana Gisele Estecho, María José Gattas, Marina Simian, María Amparo Lago Huvelle
Jazyk: angličtina
Rok vydání: 2021
Předmět:
THP-1 Cells
3D CULTURES
Cell Communication
Macrophage
Biology (General)
Spectroscopy
education.field_of_study
biology
Chemistry
Brain Neoplasms
Cell Differentiation
purl.org/becyt/ford/3.1 [https]
General Medicine
Computer Science Applications
Integrin alpha M
MONOCYTES
Monocyte differentiation
purl.org/becyt/ford/3 [https]
monocytes
MACROPHAGE POLARIZATION
tumor-stromal interactions
QH301-705.5
CD14
macrophage polarization
Population
Primary Cell Culture
Macrophage polarization
GLIOBLASTOMA MULTIFORME
Catalysis
Article
Inorganic Chemistry
Mitochondrial Proteins
glioblastoma multiforme
TUMOR-STROMAL INTERACTIONS
Cell Line
Tumor
Spheroids
Cellular
Humans
Physical and Theoretical Chemistry
education
Molecular Biology
QD1-999
3D cultures
Macrophages
Organic Chemistry
Coculture Techniques
CD206
Cell culture
Tumor progression
Culture Media
Conditioned
biology.protein
Cancer research
Carrier Proteins
Glioblastoma
Biomarkers
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 5105, p 5105 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 10
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1661-6596
1422-0067
Popis: Background: Glioblastoma multiforme (GBM) is the most frequent and aggressive primary brain tumor, and macrophages account for 30–40% of its composition. Most of these macrophages derive from bone marrow monocytes playing a crucial role in tumor progression. Unraveling the mechanisms of macrophages-GBM crosstalk in an appropriate model will contribute to the development of specific and more successful therapies. We investigated the interaction of U87MG human GBM cells with primary human CD14+ monocytes or the THP-1 cell line with the aim of establishing a physiologically relevant heterotypic culture model. Methods: primary monocytes and THP-1 cells were cultured in the presence of U87MG conditioned media or co-cultured together with previously formed GBM spheroids. Monocyte differentiation was determined by flow cytometry. Results: primary monocytes differentiate to M2 macrophages when incubated with U87MG conditioned media in 2-dimensional culture, as determined by the increased percentage of CD14+CD206+ and CD64+CD206+ populations in CD11b+ cells. Moreover, the mitochondrial protein p32/gC1qR is expressed in monocytes exposed to U87MG conditioned media. When primary CD14+ monocytes or THP-1 cells are added to previously formed GBM spheroids, both invade and establish within them. However, only primary monocytes differentiate and acquire a clear M2 phenotype characterized by the upregulation of CD206, CD163, and MERTK surface markers on the CD11b+CD14+ population and induce alterations in the sphericity of the cell cultures. Conclusion: our results present a new physiologically relevant model to study GBM/macrophage interactions in a human setting and suggest that both soluble GBM factors, as well as cell-contact dependent signals, are strong inducers of anti-inflammatory macrophages within the tumor niche. Fil: Gattas, María José. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina Fil: Estecho, Ivana Gisele. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina Fil: Lago Huvelle, María Amparo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina Fil: Errasti, Andrea Emilse. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Farmacología; Argentina. Instituto Superior de Formacion Docente y Tecnica Numero 24 Doctor Bernardo Houssay.; Argentina Fil: Carrera Silva, Eugenio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Simian, Marina. Universidad Nacional de San Martin. Instituto de Nanosistemas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Databáze: OpenAIRE
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