Development of an Aeromonas hydrophila recombinant extracellular protease vaccine
| Autor: | Chengping Lu, Lei Wu, Ya-nan Jiang, Huochun Yao, Qian Tang, Hui-xing Lin |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Recombinant Fusion Proteins
Protein subunit Microbiology law.invention Mice Plasmid law Animals Mice Inbred ICR Vaccines Synthetic Expression vector biology Immunogenicity Antibody titer biology.organism_classification Antibodies Bacterial Survival Analysis Fusion protein Virology Aeromonas hydrophila Disease Models Animal Infectious Diseases Bacterial Vaccines Vaccines Subunit Recombinant DNA Female Gram-Negative Bacterial Infections Peptide Hydrolases |
| Zdroj: | Microbial Pathogenesis. 53:183-188 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2012.07.007 |
| Popis: | Aeromonas hydrophila (Ah) exists widely in the aquatic environment and infects a variety of animals. Extracellular protease (EPR) is an important protective antigen that induces a specific antibody response to resist Ah infection. In this study, two genes encoding extracellular protease epr2 and epr3 were linked within the expression vector pET32a to construct a recombinant pET-epr2-3 plasmid. The immunogenicity of the fusion protein epr2-3 was investigated as a subunit vaccine in ICR mice. The recombinant epr2-3 protein induced the production of high antibody titers. The survival rate against homogenous Ah J-1 challenge was significantly higher in the epr2-3 vaccinated group (≥80%) compared with the inactivated Ah vaccinated group and the challenge control group (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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