Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp
| Autor: | N.F. Hepburn, D.R. Fenlon, T.H. Pennington, S.M. Rusbridge, Iain D Ogden, Marion MacRae, Norval J. C. Strachan |
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| Rok vydání: | 2002 |
| Předmět: |
Veterinary medicine
Time Factors Sorbitol-MacConkey agar Sheep Diseases Biology Escherichia coli O157 medicine.disease_cause Immunomagnetic separation Applied Microbiology and Biotechnology Pasture Disease Outbreaks Microbiology Feces chemistry.chemical_compound Most probable number medicine Animals Humans Escherichia coli Escherichia coli Infections Soil Microbiology geography Sheep geography.geographical_feature_category Immunomagnetic Separation Outbreak chemistry Cefixime medicine.drug |
| Zdroj: | Letters in Applied Microbiology. 34:100-104 |
| ISSN: | 0266-8254 |
| DOI: | 10.1046/j.1472-765x.2002.01052.x |
| Popis: | Aims: To monitor the decay of E. coli O157 in soil (loamy sand) on a scout campsite following an outbreak in humans. Methods and Results: Samples of soil and sheep faeces were collected from the campsite and tested for the presence of E. coli O157 by immunomagnetic separation (IMS) after enrichment in buffered peptone water + vancomycin at 42°C for 6 h. Enumeration of target was carried out by direct plating onto sorbitol MacConkey agar plates supplemented with cefixime and tellurite (CTSMAC) incubated at 37°C for 24 h. Low numbers ( |
| Databáze: | OpenAIRE |
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