Contribution of IgG avidity and PCR for the early diagnosis of toxoplasmosis in pregnant women from the North-Eastern region of Algeria
| Autor: | Hajira Berredjem, Meriem Benlaifa, Imène Becheker, Mohamed Reda Djebar, Hayette Aouras |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
0301 basic medicine Amniotic fluid 030106 microbiology Antibody Affinity Antibodies Protozoan serology Enzyme-Linked Immunosorbent Assay chemical and pharmacologic phenomena Polymerase Chain Reaction Serology law.invention 03 medical and health sciences Predictive Value of Tests Pregnancy law Toxoplasmosis pregnant women serology IgG avidity PCR medicine Humans Serologic Tests Avidity Polymerase chain reaction Fetus biology business.industry Articles General Medicine DNA Protozoan Igg avidity Amniotic Fluid medicine.disease Virology Toxoplasmosis Immunoglobulin A PCR Immunoglobulin M Algeria Immunoglobulin G Pregnancy Complications Parasitic Immunology IgG avidity biology.protein Female Antibody business Nucleic Acid Amplification Techniques Toxoplasma pregnant women |
| Zdroj: | African Health Sciences; Vol 17, No 3 (2017); 647-656 African Health Sciences |
| ISSN: | 1680-6905 |
| Popis: | Background: Acute toxoplasmosis in pregnant women presents a high risk of Toxoplasma transmission to the fetus. Early diagnosis is difficult, especially when serological testing for IgG/IgM antibodies fail to differentiate between a recent and a past infection. In this case, we rely on IgG avidity or PCR assays. Objectives: The aim of this study was to compare conventional ELISA and IgG avidity, with PCR using B1 and P30 primers for the early diagnosis of toxoplasmosis in pregnant women. Methods: Sera were collected from 143 pregnant women and measured by ELISA for anti- Toxoplasma IgG, IgM, IgA and IgG avidity. DNA was extracted from 57 peripheral blood and 14 amniotic fluid samples for PCR amplification. Results: A total of 57 out 143 women were seropositive: 30 (52.6%) were IgG+/IgM- and 27 (43.8%) were IgG+/IgM+; IgA antibodies were positive in 7 (12.2%) cases. IgG avidity was low in 9 women suggesting an acute infection; 3 women presented an intermediate avidity. PCR detected Toxoplasma DNA in 9 women presenting low avidity and was negative for the intermediate avidity cases. Conclusion: PCR combined to avidity IgG performed better than ELISA IgG, IgM and/or IgA assays alone. PCR was useful in the case of intermediate avidity. Keywords: Toxoplasmosis, pregnant women, serology, IgG avidity, PCR |
| Databáze: | OpenAIRE |
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