Xerocomus chrysenteron lectin: identification of a new pesticidal protein

Autor: Louis Chavant, Véronique Trigueros, Laurent Paquereau, Djamila Ali-Ahmed, Andrée Lougarre, Yvan Rahbé, J. Guillot, Didier Fournier
Přispěvatelé: Institut de pharmacologie et de biologie structurale (IPBS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Biologie Fonctionnelle, Insectes et Interactions (BF2I), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA), Faculté de Pharmacie - Clermont-Ferrand, Université d'Auvergne - Clermont-Ferrand I (UdA), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées
Jazyk: angličtina
Rok vydání: 2003
Předmět:
Insecticides
MESH: Amino Acid Sequence
MESH: Base Sequence
medicine.disease_cause
Biochemistry
Xerocomus
MESH: Lectins
Lectins
MESH: Animals
Cloning
Molecular
0303 health sciences
Fungal protein
Mushroom
biology
MESH: Peptides
030302 biochemistry & molecular biology
Edible mushroom
Drosophila melanogaster
Fungal
MESH: Fungal Proteins
Genes
Fungal
Molecular Sequence Data
Biophysics
MESH: Sequence Alignment
MESH: Drosophila melanogaster
Fungal Proteins
03 medical and health sciences
Complementary DNA
Toxicity Tests
medicine
Animals
MESH: Cloning
Molecular
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Amino Acid Sequence
MESH: Toxicity Tests
Molecular Biology
Escherichia coli
030304 developmental biology
MESH: Molecular Sequence Data
Base Sequence
Basidiomycota
fungi
Lectin
Molecular
biology.organism_classification
MESH: Insecticides
MESH: Basidiomycota
Genes
biology.protein
Peptides
MESH: Genes
Fungal
Sequence Alignment
Galanthus nivalis
Cloning
Zdroj: Biochimica et Biophysica Acta-Molecular Cell Research
Biochimica et Biophysica Acta-Molecular Cell Research, Elsevier, 2003, 1621 (3), pp.292-298. ⟨10.1016/S0304-4165(03)00098-9⟩
Biochimica et Biophysica Acta-Molecular Cell Research, 2003, 1621 (3), pp.292-298. ⟨10.1016/S0304-4165(03)00098-9⟩
ISSN: 0167-4889
Popis: Xerocomus chrysenteron is an edible mushroom with insecticidal properties. In an earlier work, we found that proteins are responsible for this toxicity. Here we describe the purification of a approximately 15 kDa lectin, named XCL, from the mushroom. Its cDNA and gDNA were cloned by PCR strategies and a recombinant form was expressed in Escherichia coli. Sequence alignments and sugar specificity showed that this protein is the third member of a new saline-soluble lectin family present in fungi. This protein, either purified from mushroom or expressed in vitro in E. coli, was found to be toxic to some insects, such as the dipteran Drosophila melanogaster and the hemipteran, Acyrthosiphon pisum. The lectin possesses a high insecticidal activity compared to lectin isolated from leguminosae (Lathyrus ochrus) or from the snowdrop (Galanthus nivalis).
Databáze: OpenAIRE
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