The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion
| Autor: | Mustafa Orkmez, Davut Sinan Kaplan, Beyhan Cengiz Bağci, Abdulkadir Yasir Bahar, Tuncer Demir, Recep Dokuyucu, Ayse Binnur Erbagci, Yumrutaş Onder, Beyhan Cengiz, Ibrahim Bozgeyik, Recep Bayraktar |
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| Přispěvatelé: | Hatay Mustafa Kemal Üniversitesi, Dokuyucu, R, Demir, T, Yumrutas, O, Erbagci, AB, Orkmez, M, Bahar, AY, Bayraktar, R, Bozgeyik, I, Kaplan, DS, Cengiz, B, Bagci, C, Sakarya Üniversitesi/Tıp Fakültesi/Temel Tıp Bilimleri Bölümü, Bağcı, Cahit |
| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology medicine.medical_specialty Growth Differentiation Factor 15 Bone Morphogenetic Protein 6 H&E stain Ischemia GPI-Linked Proteins Kidney Hepcidins Hepcidin General & Internal Medicine Internal medicine Animals Medicine Rats Wistar Hemochromatosis Protein Cerrahi Hemojuvelin biology Renal ischemia Reverse Transcriptase Polymerase Chain Reaction business.industry Membrane Proteins Kidney metabolism General Medicine medicine.disease Disease Models Animal medicine.anatomical_structure Endocrinology Reperfusion Injury biology.protein GDF15 business |
| Zdroj: | TURKISH JOURNAL OF MEDICAL SCIENCES. 44:576-581 |
| ISSN: | 1303-6165 1300-0144 |
| DOI: | 10.3906/sag-1307-100 |
| Popis: | Background/aim: To determine the roles of hepcidin and its related genes in a renal ischemia/reperfusion model. Materials and methods: A total of 20 Wistar albino rats were equally divided into 2 groups: Group I was the control group and Group II was the ischemia and reperfusion (I/R) group (60 min of ischemia + 48 h of reperfusion). I/R was performed on the left kidneys of these rats and then the I/R-treated kidneys were removed. The levels of serum biochemical markers were evaluated after renal I/R. The expression levels of hepcidin-linked genes [growth differentiation factor 15 (GDF-15), bone morphogenetic protein 6 (BMP6), and hemojuvelin (HJV)] were also measured by RT-PCR technique. In addition, the tissues were evaluated histopathologically. Results: No significant association was found between renal dysfunction and I/R when compared to biochemical parameters (P > 0.05). However, differences in platelet values were statistically significant (P < 0.05). Expression levels of GDF-15, BMP6, and HJV genes increased, but this increase was not statistically significant. In addition, histopathological evaluation was performed using hematoxylin and eosin stain. This showed a significant relationship between the control group and I/R group for ischemic and nonischemic kidney scoring. Conclusion: Hepcidin and BMP6, HJV, and GDF-15 should be taken into account when investigating the process of I/R. |
| Databáze: | OpenAIRE |
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