Characterization and biological activity of the main flavonoids from Swiss Chard (Beta vulgaris subspecies cycla)
| Autor: | Giovanni Piccoli, A.M. Di Gioacchino, Antonella Antonelli, Vilberto Stocchi, Mara Bacchiocca, Paolino Ninfali, Enrica Biagiotti, Giorgio Brandi |
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| Rok vydání: | 2006 |
| Předmět: |
Pharmaceutical Science
Breast Neoplasms Biology Antioxidants chemistry.chemical_compound Rutin Inhibitory Concentration 50 food Cell Line Tumor Drug Discovery Humans Isorhamnetin Pharmacology Flavonoids Dose-Response Relationship Drug Cell growth Plant Extracts Biological activity Swiss Chard Drug Synergism Antineoplastic Agents Phytogenic food.food Complementary and alternative medicine chemistry Biochemistry Apoptosis Cell culture Toxicity Molecular Medicine Female Beta vulgaris Phytotherapy |
| Zdroj: | Phytomedicine : international journal of phytotherapy and phytopharmacology. 14(2-3) |
| ISSN: | 0944-7113 |
| Popis: | The molecular components of a phenolic fraction (P2), obtained from liquid chromatography of a Swiss Chard (Beta vulgaris subsp. cycla) extract, were identified using HPLC-ESI-MS/MS. The primary P2 components were: vitexin-2''O-rhamnoside, its demethylated form 2''-xylosylvitexin, isorhamnetin 3-gentiobioside, and rutin. P2 "in toto" and the single components were characterized for antioxidant capacity, antimitotic activity on MCF-7 human breast cancer cells and for toxicity to human lymphocytes and macrophages. P2 inhibited MCF-7 cell proliferation (IC(50) value = 9 microg/ml) without inducing apoptosis, showed no toxicity to human lymphocytes and slight toxicity to macrophages. Vitexin-2''O-rhamnoside strongly inhibited DNA synthesis in MCF-7 cells, whereas 2''-xylosylvitexin and isorhamnetin 3-gentiobioside were activators; combinations of activators and inhibitors maintained the over-all inhibitory effect. |
| Databáze: | OpenAIRE |
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