OTK18, a zinc-finger protein, regulates human immunodeficiency virus type 1 long terminal repeat through two distinct regulatory regions
| Autor: | Jenae Limoges, Masahide Horiba, Yunquan Jiang, Sinji Sato, Tsuneya Ikezu, James L. Buescher, Lindsey B. Martinez, Clinton Jones |
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| Rok vydání: | 2006 |
| Předmět: |
Zinc finger
Gene Expression Regulation Viral Transcription Genetic viruses Mutant Biology DNA-binding protein Virology Molecular biology Long terminal repeat Virus Article DNA-Binding Proteins Regulatory sequence DNA Viral HIV-1 Humans HIV Long Terminal Repeat Transcription factor Transcription Factors |
| Zdroj: | The Journal of general virology. 88(Pt 1) |
| ISSN: | 0022-1317 |
| Popis: | It has previously been shown by our laboratory that OTK18, a human immunodeficiency virus (HIV)-inducible zinc-finger protein, reduces progeny-virion production in infected human macrophages. OTK18 antiviral activity is mediated through suppression of Tat-induced HIV-1 long terminal repeat (LTR) promoter activity. Through the use of LTR-scanning mutant vectors, the specific regions responsible for OTK18-mediated LTR suppression have been defined. Two different LTR regions were identified as potential OTK18-binding sites by an enhanced DNA–transcription factor ELISA system; the negative-regulatory element (NRE) at −255/−238 and the Ets-binding site (EBS) at −150/−139 in the LTR. In addition, deletion of the EBS in the LTR blocked OTK18-mediated LTR suppression. These data indicate that OTK18 suppresses LTR activity through two distinct regulatory elements. Spontaneous mutations in these regions might enable HIV-1 to escape from OTK18 antiretroviral activity in human macrophages. |
| Databáze: | OpenAIRE |
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