DNA Polymerase Delta Synthesizes Both Strands during Break-Induced Replication
| Autor: | Zhi-Xiong Zhou, Roberto A. Donnianni, Eleanor Glancy, Valerie E Garcia, Thomas A. Kunkel, Adam B. Burkholder, Scott A. Lujan, Amr Al-Zain, Lorraine S. Symington |
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| Rok vydání: | 2018 |
| Předmět: |
DNA Replication
Saccharomyces cerevisiae Proteins DNA polymerase DNA repair Saccharomyces cerevisiae DNA polymerase delta Article 03 medical and health sciences DNA Ligase ATP 0302 clinical medicine Complementary DNA Humans DNA Fungal Molecular Biology Polymerase 030304 developmental biology DNA Polymerase III 0303 health sciences biology DNA synthesis DNA Breaks Cell Biology DNA Polymerase II DNA Polymerase I Cell biology Telomere HEK293 Cells biology.protein Homologous recombination 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | Molecular cell. 76(3) |
| ISSN: | 1097-4164 |
| Popis: | Break-induced replication (BIR) is a pathway of homology-directed repair that repairs one-ended DNA breaks, such as those formed at broken replication forks or uncapped telomeres. In contrast to conventional S phase DNA synthesis, BIR proceeds by a migrating D-loop and results in conservative synthesis of the nascent strands. DNA polymerase delta (Pol δ) initiates BIR; however, it is not known whether synthesis of the invading strand switches to a different polymerase or how the complementary strand is synthesized. By using alleles of the replicative DNA polymerases that are permissive for ribonucleotide incorporation, thus generating a signature of their action in the genome that can be identified by hydrolytic end sequencing, we show that Pol δ replicates both the invading and the complementary strand during BIR. In support of this conclusion, we show that depletion of Pol δ from cells reduces BIR, whereas depletion of Pol e has no effect. |
| Databáze: | OpenAIRE |
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