Role of prostaglandin E-2 in the modulation of Wnt canonical signaling in cells on microstructured titanium surfaces
Autor: | Elena Calciolari, Antonio Cacchioli, Federico Rivara, Simone Lumetti, Edoardo Manfredi, Guido Maria Macaluso, Andrea Toffoli, Carlo Galli, Francesca Ravanetti, G. Ghiacci |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
Surface Properties
Cellular differentiation 0206 medical engineering Biomedical Engineering Biophysics Bioengineering 02 engineering and technology Cell morphology Dinoprostone Cell Line Biomaterials Focal adhesion Wnt signaling pathway 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Prostaglandin E2 Cell adhesion Titanium Osteoblasts Chemistry Osteoblast Cell Differentiation 030206 dentistry General Medicine Anatomy 020601 biomedical engineering Antigens Differentiation Cell biology medicine.anatomical_structure Gene Expression Regulation Prostaglandins C2C12 medicine.drug |
Zdroj: | J Appl Biomater Function Mater 14 (2016): E181–E188. doi:10.5301/jabfm.5000267 info:cnr-pdr/source/autori:Manfredi, Edoardo; Lumetti, Simone; Rivara, Federico; Toffoli, Andrea; Calciolari, Elena; Cacchioli, Antonio; Ravanetti, Francesca; Ghiacci, Giulia; Macaluso, Guido; Galli, Carlo/titolo:Role of prostaglandin E-2 in the modulation of Wnt canonical signaling in cells on microstructured titanium surfaces/doi:10.5301%2Fjabfm.5000267/rivista:J Appl Biomater Function Mater/anno:2016/pagina_da:E181/pagina_a:E188/intervallo_pagine:E181–E188/volume:14 |
Popis: | Background: Rough surface topography enhances the activation of Wnt canonical signaling, a pathway required for osteoblast differentiation. The present study investigated the effects of the modulation of prostaglandin E-2 (PGE(2)) signaling on osteoblastic differentiation on titanium surfaces for endosseous implants with different topographies. Methods: C2C12 cells were plated on polished or acid-etched/sand-blasted (SLA) titanium discs and stimulated with 1 mu M PGE(2) or 100 nM cyclooxygenase inhibitor indomethacin. Activation of Wnt canonical signaling was measured with a reporter system. Gene expression was measured in the same cell system by real-time polymerase chain reaction (RT-PCR). Osteoblastic MC3T3 cells were then plated on polished or SLA titanium discs with or without indomethacin, and their proliferation and the expression of osteoblast-specific genes was assessed by RT-PCR. Cell morphology was furthermore studied on SEM, and cell adhesion was assessed by fluorescent labeling of focal adhesion. Results: PGE(2) decreased Wnt signaling stimulation in cells growing on polished or SLA surfaces, while indomethacin increased the expression of Wnt target genes in C2C12 and MC3T3 cells, by reporter assay. Moreover, indomethacin increased the expression of early differentiation marker alkaline phosphatase in MC3T3 cells on polished discs and of late marker osteocalcin in cells on SLA titanium. Conclusions: Prostaglandin signaling affects the activation of Wnt canonical pathway in osteoblastic and mesenchymal cells on microstructured surfaces. |
Databáze: | OpenAIRE |
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