Using zebrafish embryo bioassays combined with high-resolution mass spectrometry screening to assess ecotoxicological water bodies quality status: A case study in Panama rivers
| Autor: | Verónica Castro, Maria Natividade Vieira, Rosario Rodil, Raquel S. Chaves, Miguel M. Santos, Miguel Espinosa, José Benito Quintana, Estibali Wilkie Wilson |
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| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía, Universidade de Santiago de Compostela. Instituto de Investigación e Análises Alimentarias, Repositório da Universidade de Lisboa |
| Rok vydání: | 2021 |
| Předmět: |
animal structures
Environmental Engineering Panama Health Toxicology and Mutagenesis 0208 environmental biotechnology Anthropogenic pressure 02 engineering and technology 010501 environmental sciences Biology Positive correlation 01 natural sciences Mass Spectrometry Rivers Animals Environmental Chemistry Bioassay Zebrafish Risk assessment 0105 earth and related environmental sciences Emerging contaminants Danio rerio Public Health Environmental and Occupational Health Water General Medicine General Chemistry Contamination Pesticide Quadrupole-time-of flight mass spectrometry (QTOF) Pollution 020801 environmental engineering Environmental chemistry Screening Toxicity testing Zebrafish embryo Biological Assay Water Pollutants Chemical Environmental Monitoring |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname |
| ISSN: | 0045-6535 2017-8476 |
| DOI: | 10.1016/j.chemosphere.2021.129823 |
| Popis: | Copyright © 2021 Elsevier Ltd. All rights reserved. Several studies show that many water bodies in developing countries are increasingly affected by anthropogenic pressure, such as agricultural activities, domestic and industrial wastewater. However, data is scarce in several of such countries, including Panama. Thus, in this work, the ecotoxicological status of selected rivers in Panama with distinct input sources were evaluated using the zebrafish (Danio rerio) embryo bioassays combined with a liquid chromatography-high resolution mass spectrometry screening of contaminants of emerging concern (CECs), using a library of over 3200 chemicals. A total of 68 CECs, including pharmaceuticals and metabolites, pesticides and several industrial chemicals, could be tentatively identified. Additionally, the zebrafish embryo bioassays showed a significant increase (p < 0.05) in embryo mortality/abnormalities when incubated with water samples from two rivers, Matasnillo and Curundú (47.5% and 32%, respectively). Importantly, a positive correlation between ecotoxicological endpoints and some of the detected CECs was observed. The findings demonstrate that both rivers are under strong anthropogenic pressure, and therefore, management actions are urgently needed to decrease their level of contamination. Overall, this study further supports the use of the zebrafish embryo bioassay as a fast, high throughput approach for screening the toxicity of water samples, and highlights the advantages of combining ecotoxicological assays with high-resolution mass spectrometry to an expedite assessment of the ecotoxicological status of water bodies. This work was supported by the Portuguese Foundation for Science and Technology (FCT) (PTDC|CTA-AMB|31554|2017; UIDB/04423/2020; UIDP/04423/2020), the Spanish Agencia Estatal de Investigacion (ref. CTM2017-84763-C3-2-R), the Galician Council of Culture, Education and Universities (ref. ED431C2017/36 and Veronica Castro predoctoral contract, ref. ED481A-2017/156), co-funded by the European Regional Development Fund (ERDF/FEDER). |
| Databáze: | OpenAIRE |
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