NOD2 mediates isoflurane preconditioning-induced protection of myocardial injury
| Autor: | Cheng Yang, Yiqing Ren, Nuo Yan, Jinchao Sun, Jie Gao, Banglin Wu, Hui Li, Yang Jiao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Autophagosome Myocardial Infarction Nod2 Signaling Adaptor Protein FOXO1 Pharmacology Biology p38 Mitogen-Activated Protein Kinases Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine Animals Myocytes Cardiac Viability assay Phosphorylation Protein kinase A Cardioprotection Isoflurane General Neuroscience Autophagy 030104 developmental biology Animals Newborn Biochemistry Reperfusion Injury 030220 oncology & carcinogenesis Anesthetics Inhalation Anesthetic Mitogen-Activated Protein Kinases Signal Transduction medicine.drug |
| Zdroj: | Neuroscience Letters. 637:154-160 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2016.11.031 |
| Popis: | Anesthetic cardioprotection reduces myocardial infarct size following ischemia-reperfusion injury. However, the underlying mechanisms that drive ischemia-reperfusion injury in cardiomyocytes remain unclear. In this study, we report that isoflurane, a commonly used inhaled anesthetic, can protect cardiomyocytes from anoxia/reoxygenation injury by a nucleotide binding oligomerization domain containing 2 (NOD2)-dependent mechanism. The results showed that isoflurane increased cell viability, and decreased autophagosome generation in primary cardiomyocytes under anoxia/reoxygenation conditions. In addition, western blot revealed that isoflurane reduces the expression of NOD2. Overexpression of NOD2 is accompanied by an increased expression of autophagy-related genes, decreased cell viability, and enhanced expression of phosphorylation p38-mitogen-activated protein kinase (p38MAPK), while NOD2 knockdown exerted the opposite effect. Following preconditioning with SB203580, a p38MAPK inhibitor, the inhibitory effect of isoflurane on cardiomyocytes autophagy was further enhanced, which suggests that p38MAPK is involved in the mechanism of cardioprotection provided by isoflurane. These findings reveal a novel mechanism underlying isoflurane-afford protection of myocardial injury. |
| Databáze: | OpenAIRE |
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