circAMOTL1L Suppresses Renal Cell Carcinoma Growth by Modulating the miR-92a-2-5p/KLLN Pathway
| Autor: | Ling Gao, Ruonan Zhang, Weifei Wu, Fujun Hou, Xiaolei He, Limin Li, Qingqing Yue, Xuejuan Yang, Xian Shao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Aging Article Subject Down-Regulation Biology urologic and male genital diseases Biochemistry Metastasis Downregulation and upregulation Cell Movement Renal cell carcinoma Cell Line Tumor medicine Animals Humans Luciferase Carcinoma Renal Cell Aged Cell Proliferation Mice Inbred BALB C QH573-671 Cell growth Tumor Suppressor Proteins RNA Circular Cell Biology General Medicine Middle Aged medicine.disease Xenograft Model Antitumor Assays Kidney Neoplasms female genital diseases and pregnancy complications Gene Expression Regulation Neoplastic MicroRNAs Cell culture Apoptosis Tumor progression Cancer research Female Cytology Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2021 (2021) Oxidative Medicine and Cellular Longevity |
| ISSN: | 1942-0994 1942-0900 |
| Popis: | Accumulating evidence indicates that the dysregulation of circular RNAs (circRNAs) contributes to tumor progression; however, the regulatory functions of circRNAs in renal cell carcinoma (RCC) remain largely unknown. In this study, the function and underlying mechanism of circAMOTL1L in RCC progression were explored. qRT-PCR showed the downregulation of circAMOTL1L in RCC tissues and cell lines. The decrease in circAMOTL1L expression correlated with the tumor stage, metastasis, and poor prognosis in patients with RCC. Functional experiments revealed that circAMOTL1L inhibited cell proliferation and increased apoptosis in RCC cells. Subcutaneous implantation with circAMOTL1L-overexpressing cells in nude mice decreased the growth ability of the xenograft tumors. Mechanistically, circAMOTL1L served as a sponge for miR-92a-2-5p in upregulating KLLN (killin, p53-regulated DNA replication inhibitor) expression validated by bioinformatics analysis, oligo pull-down, and luciferase assays. Further, reinforcing the circAMOTL1L–miR-92a-2-5p–KLLN axis greatly reduced the growth of RCC in vivo. Conclusively, our findings demonstrate that circAMOTL1L has an antioncogenic role in RCC growth by modulating the miR-92a-2-5p–KLLN pathway. Thus, targeting the novel circAMOTL1L–miR-92a-2-5p–KLLN regulatory axis might provide a therapeutic strategy for RCC. |
| Databáze: | OpenAIRE |
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