IVIg modulates BCR signaling through CD22 and promotes apoptosis in mature human B lymphocytes
| Autor: | Pierre Youinou, Yves Renaudineau, Rizgar A. Mageed, Divi Cornec, Sophie Hillion, Jean-François Séité |
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| Přispěvatelé: | Immunologie et Pathologie (EA2216), Université de Brest (UBO)-IFR148, CHRU Brest - Service de Rhumatologie (CHU - BREST - Rhumato), Centre Hospitalier Régional Universitaire de Brest (CHRU Brest), Laboratoire d'Immunologie et Immunothérapie, William Harvey Research Institute, Barts and the London Medical School, LabEX IGO Immunothérapie Grand Ouest |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
MAPK/ERK pathway
MESH: Signal Transduction Time Factors Sialic Acid Binding Ig-like Lectin 2 Apoptosis MESH: Flow Cytometry MESH: Cell Cycle Biochemistry MESH: Dose-Response Relationship Drug chemistry.chemical_compound 0302 clinical medicine hemic and lymphatic diseases MESH: Child MESH: Microscopy Confocal MESH: Immunoglobulins Intravenous Child Receptor Cells Cultured Mitogen-Activated Protein Kinase 1 B-Lymphocytes 0303 health sciences Microscopy Confocal Mitogen-Activated Protein Kinase 3 biology MESH: Immunologic Factors Cell Cycle CD22 Immunoglobulins Intravenous Hematology Cell cycle Flow Cytometry 3. Good health Cell biology MESH: Cell Survival MESH: Sialic Acid Binding Ig-like Lectin 2 [SDV.IMM]Life Sciences [q-bio]/Immunology Antibody Signal transduction MESH: N-Acetylneuraminic Acid MESH: Mitogen-Activated Protein Kinase 3 Protein Binding Signal Transduction MESH: Cells Cultured MESH: Mitogen-Activated Protein Kinase 1 MESH: Enzyme Activation MESH: Cell Line Tumor Cell Survival Blotting Western Immunology Receptors Antigen B-Cell MESH: Receptors Antigen B-Cell 03 medical and health sciences LYN Cell Line Tumor MESH: B-Lymphocytes Humans Immunologic Factors MESH: Protein Binding MESH: Blotting Western 030304 developmental biology MESH: Humans Dose-Response Relationship Drug MESH: Apoptosis MESH: Time Factors Tyrosine phosphorylation Cell Biology N-Acetylneuraminic Acid Enzyme Activation chemistry biology.protein 030215 immunology |
| Zdroj: | Blood Blood, American Society of Hematology, 2010, 116 (10), pp.1698-704. ⟨10.1182/blood-2009-12-261461⟩ |
| ISSN: | 0006-4971 1528-0020 |
| DOI: | 10.1182/blood-2009-12-261461⟩ |
| Popis: | Among various mechanisms for interactions with B cells, intravenous immunoglobulin (IVIg) may operate through the insertion of its Fc part into the Fc-γ receptor, or the binding of its sialic acid (SA)–bearing glycans to the negatively regulating CD22 lectin. It appeared that IVIg reduces B lymphocyte viability in a dose- and time-dependent manner. Furthermore, we show by confocal microscopy that SA-positive IgG, but not SA-negative IgG bind to CD22. This interaction reduces the strength of B-cell receptor–mediated signaling trough down-regulating tyrosine phosphorylation of Lyn and the B-cell linker proteins, and up-regulating phospholipase Cγ2 activation. This cascade resulted in a sustained activation of Erk 1/2 and arrest of the cell cycle at the G1 phase. These changes may be accounted for the efficacy of IVIg in autoimmune diseases. |
| Databáze: | OpenAIRE |
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