Conversion of pBR322-based plasmids into broad-host-range vectors by using the Tn3 transposition mechanism
| Autor: | M Rekik, Shigeaki Harayama, B Witholt, M Kok |
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| Rok vydání: | 1994 |
| Předmět: |
Transposable element
Genetic Vectors Lactose Biology medicine.disease_cause Microbiology law.invention Transposition (music) Plasmid Species Specificity law Alkanes Gram-Negative Bacteria Operon Escherichia coli medicine Vector (molecular biology) Cloning Molecular Molecular Biology Recombination Genetic Genetics Pseudomonas putida biology.organism_classification PBR322 Biodegradation Environmental DNA Transposable Elements Recombinant DNA Research Article |
| Zdroj: | Journal of Bacteriology. 176:6566-6571 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.176.21.6566-6571.1994 |
| Popis: | We constructed a series of transposon vectors which allow efficient in vitro gene manipulation and subsequent introduction of cloned DNA into a variety of gram-negative bacteria. Transfer of the cloned fragment from these multicopy plasmids into self-transmissible broad-host-range vectors is achieved in vivo, using the Tn3 transposition mechanism. Transposition into a variety of broad-host-range plasmids proceeds efficiently, and the resulting recombinant plasmids can be readily transferred and maintained in a variety of gram-negative bacteria. The utility of the transposable vectors was demonstrated by the introduction and expression of the lacIPOZY sequences of Escherichia coli into Pseudomonas putida strains, allowing them to utilize lactose as a sole source of carbon and energy. |
| Databáze: | OpenAIRE |
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