Effect of Harvest Age on Total Phenolic, Total Anthocyanin Content, Bioactive Antioxidant Capacity and Antiproliferation of Black and White Glutinous Rice Sprouts
| Autor: | Natthida Weerapreeyakul, Visessakseth So, Benjabhorn Sethabouppha, Piman Pocasap, Jintanaporn Wattanathorn, Wipawee Thukham-mee, Khaetthareeya Sutthanut |
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| Rok vydání: | 2020 |
| Předmět: |
antiproliferative activity
Neutral red DPPH black glutinous rice lcsh:Technology Jurkat cells lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine white glutinous rice General Materials Science Gallic acid Food science lcsh:QH301-705.5 Instrumentation IC50 030304 developmental biology reactive oxygen species Fluid Flow and Transfer Processes chemistry.chemical_classification 0303 health sciences Reactive oxygen species Keywords: cancer lcsh:T Process Chemistry and Technology General Engineering food and beverages Glutathione lcsh:QC1-999 Computer Science Applications antioxidants lcsh:Biology (General) lcsh:QD1-999 chemistry lcsh:TA1-2040 030220 oncology & carcinogenesis Anthocyanin lcsh:Engineering (General). Civil engineering (General) lcsh:Physics |
| Zdroj: | Applied Sciences Volume 10 Issue 20 Applied Sciences, Vol 10, Iss 7051, p 7051 (2020) |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app10207051 |
| Popis: | Black (cv. BGR) and white (cv. RD6) glutinous rice sprouts from fertilizer- and pesticide-free farm in Khon Kaen province, Thailand were investigated for antioxidation and antiproliferative activity. Three different ages of rice sprouts were collected and prepared as the extract. BGR exerted higher antioxidant capacity than RD6 based on total phenolic (TPC) and total anthocyanin contents (TAC), DPPH, and FRAP assays. BGR at 10&ndash 15 days contained the highest TPC (29.72 ± 1.42 mg gallic acid equivalent/g extract) and reducing power (2.22 ± 0.014 mmole FeSO4/g extract). BGR at 20&ndash 25 days contained the highest TAC (0.86 ± 0.096 equivalence of cyanidin-3-glucoside/g extract) and DPPH radical scavenging activity (IC50 = 231.09 ± 12.99 &mu g/mL). Antiproliferative activity of the extracts was evaluated in the human T-lymphocyte (Jurkat), hepatocellular carcinoma (HepG2), colorectal carcinoma (HCT116), melanoma (SK-MEL-2) and noncancerous cells (Vero) by neutral red assay. BGR showed the most selective antiproliferation against Jurkat cells, by inducing apoptosis, and caspase 3/7 activity. BGR at 200 &mu g/mL from all ages significantly decreased ROS using DCFH-DA and increased endogenous glutathione levels in Jurkat cells compared to the control (p < 0.05). The higher antiproliferation of BGR than RD6 was via its antioxidation capacity and attributed to its higher phenolic and anthocyanin contents. BGR sprout is a potential source of biologically active substances good for wellness and health benefits. |
| Databáze: | OpenAIRE |
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