Loranthus acaciae: Alternative medicine for β-lactamase producer and methicillin-resistant Staphylococcus aureus

Autor: Ehssan H. Moglad
Rok vydání: 2021
Předmět:
0106 biological sciences
0301 basic medicine
MIC
minimum inhibitory concentration
Antimicrobial activities
medicine.drug_class
Antibiotics
MRSA
Biology
medicine.disease_cause
01 natural sciences
DCM
Dichloromethane
03 medical and health sciences
Minimum inhibitory concentration
MBC
bactericidal concentration
LA
Loranthus acaciae
CLSI
clinical and laboratory standards institute
Ampicillin
medicine
Loranthus acaciae
lcsh:QH301-705.5
β-lactamase enzyme
Multi-drug resistant
Antibacterial agent
Traditional medicine
MRSA
methicillin-resistant Staphylococcus aureus
ATCC
American type culture collection
Plicosepalus acaciae
Antimicrobial
Blac
β-lactamase producer
Methicillin-resistant Staphylococcus aureus
Penicillin
030104 developmental biology
lcsh:Biology (General)
Staphylococcus aureus
Original Article
SA
Staphylococcus aureus
General Agricultural and Biological Sciences
MeOH
methanol
010606 plant biology & botany
medicine.drug
Zdroj: Saudi Journal of Biological Sciences
Saudi Journal of Biological Sciences, Vol 28, Iss 3, Pp 1835-1839 (2021)
ISSN: 1319-562X
DOI: 10.1016/j.sjbs.2020.12.029
Popis: Recently, we reported high antibacterial efficiency of Loranthus acaciae (LA) against different standard strains of bacteria including Methicillin-Resistant Staphylococcus aureus (MRSA). Therefore, this study aimed to confirm the effectiveness of LA against clinically isolated Staphylococcus aureus (SA) including β-lactamase producer (Blac) and MRSA. Forty-eight SA isolates collected from various clinical samples were used in this study. Antibiotics susceptibility profile was determined for twenty different antibiotics using automated Microscan Walkaway 96 Plus system as recommended by Clinical and Laboratory Standards Institute (CLSI) guidelines. This system also identified β-lactamase producers and MRSA. In the meantime, LA ethanolic extract was fractionated using liquid–liquid fraction method to hexane, dichloromethane DCM and methanol 80% fractions. Antimicrobial activities of LA extract and fraction were performed with agar well diffusion method for all SA isolates, MIC and MBC were also recorded. Phytochemical screening for various phyto-constituent classes of LA ethanolic extract was determined. Out of 48 SA isolates, Cefoxitin-positive MRSA represent 31 (64.6%), Blac 17 (35.4%), and 41 (85.4%) were multidrug-resistant SA, which was resistant at least to one antibiotic from three different categories. All isolates were resistant to ampicillin and penicillin. Antimicrobial activities of LA extract and fractions revealed that ethanol extract was active against all isolated SA with inhibition zone ranged from 33 ± 2.00 to 25 ± 3.05 mm. While DCM exhibited the largest inhibition zone range from 37 ± 3.00 to 33 ± 2.00 mm. This study is first of its kind conforming the high antibacterial activity of LA against SA isolated from a different source of infection. The study concluded that LA extract and fractions are active and give positive result for all isolated SA. Therefore, suitable pharmacological formulation of LA extract as a promising antibacterial agent for the treatment of SA infection should be given extreme priority.
Databáze: OpenAIRE
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