Comparison of blood ethanol stabilities in different storage periods
| Autor: | Fatma Emel Koçak, Ayfer Meral, Havva Koçak, Ozben Ozden Isiklar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Automobile Driving Time Factors Wilcoxon signed-rank test Clinical Biochemistry preanalytical phase ethanol stability storage temperature Total error Toxicology chemistry.chemical_compound Animal science Proficiency testing Humans Cryopreservation Ethanol Plasma samples Chemistry Biochemistry (medical) Reproducibility of Results Blood ethanol Ethanol measurement Correlation analysis Female Alcoholic Intoxication Research Article |
| Zdroj: | Biochemia Medica Volume 25 Issue 1 |
| ISSN: | 1846-7482 1330-0962 |
| Popis: | Introduction: Measurements of blood ethanol concentrations must be accurate and reliable. The most important factors affecting blood ethanol stability are temperature and storage time. In this study, we aimed to compare ethanol stability in plasma samples at-20 °C for the different storage periods. Materials and methods: Blood samples were collected from intoxicated drivers (N = 80) and initial plasma ethanol concentrations were measured immediately. Plasma samples were then stored at-20 °C and re-assessed after 2, 3, 4, or 5 months of storage. Differences between the initial and stored ethanol concentrations in each group (N = 20) were analyzed using Wilcoxon matched-pairs test. The deviation from the initial concentration was calculated and compared with Clinical Laboratory Improvement Amendments (CLIA’88) Proficiency Testing Limits. Relationships between the initial concentrations and deviations from initial concentrations were analyzed by Spearman’s correlation analysis. For all statistical tests, differences with P values of less than 0.05 were considered statistically significant. Results: Statistically significant differences were observed between the initial and poststorage ethanol concentrations in the overall sample group (P < 0.001). However, for the individual storage duration groups, analytically significant decreases were observed only for samples stored for 5 months, deviations from the initial concentrations exceeded the allowable total error (TEa). Ethanol decreases in the other groups did not exceed the TEa. Conclusion: According to our results, plasma ethanol samples can be kept at-20 °C for up to 3-4 months until re-analysis. However, each laboratory should also establish its own work-flow rules and criterion for reliable ethanol measurement in forensic cases. © by Croatian Society of Medical Biochemistry and Laboratory Medicine. |
| Databáze: | OpenAIRE |
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