Immunohistochemistry of the intercellular matrix components and the epithelio-mesenchymal junction of the human tooth germ
| Autor: | C. Garbarsch, Dennis Moe, M. E. Matthiessen, Svend Kirkeby, Birgitte Engelbrecht Olsen |
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| Rok vydání: | 1994 |
| Předmět: |
Cell Adhesion Molecules
Neuronal Tenascin Extracellular matrix Fetus stomatognathic system Laminin Human tooth Ameloblasts medicine Dentin Humans Dental Papilla Extracellular Matrix Proteins biology Enamel paint Chemistry Tooth Germ Cell Biology Anatomy Immunohistochemistry Fibronectins Cell biology Fibronectin stomatognathic diseases Collagen type I alpha 1 medicine.anatomical_structure visual_art biology.protein visual_art.visual_art_medium Odontogenesis Collagen Heparitin Sulfate |
| Zdroj: | The Histochemical Journal. 26:110-118 |
| ISSN: | 1573-6865 0018-2214 |
| DOI: | 10.1007/bf00157959 |
| Popis: | The immunohistochemical localization of heparan sulphate, collagen type I, III and IV, laminin, tenascin, plasma- and cellular fibronectin was studied in tooth germs from human fetuses. The lamina basalis ameloblastica or membrana preformativa, which separates the pre-ameloblasts from the pre-dentin and dentin, contained heparan sulphate, collagen type IV, laminin and fibronectin. Enamel reacted with antifibronectin, but the reaction varied depending on the type of fibronectin and the source of antibody. In early pre-dentin, collagen type I, laminin, tenascin and fibronectin were present. In late pre-dentin and dentin collagen type I was found in intertubular dentin and in the zone between enamel and dentin. The close relationship between collagen type I in dentin and fibronectin in immature enamel is interesting, as it may contribute to the stabilization of the amelodentinal interface. In dental pulp, collagen type IV and laminin were found in the endothelial basement membranes. Collagen type I and III, tenascin and fibronectin were localized to the mesenchymal intercellular matrix. The results of this study have supported the assumption that the lamina basalis ameloblastica is a basement membrane, and have lead to the suggestion that ameloblasts are producers of fibronectin or a fibronectin-like substance. |
| Databáze: | OpenAIRE |
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