Gallic acid modulates purine metabolism and oxidative stress induced by ethanol exposure in zebrafish brain

Autor: Samira Leila Baldin, Karolyne de Pieri Pickler, Ana Caroline Salvador de Farias, Henrique Teza Bernardo, Rahisa Scussel, Bárbara da Costa Pereira, Suzielen Damin Pacheco, Eduardo Ronconi Dondossola, Ricardo Andrez Machado-de-Ávila, Almir Gonçalves Wanderley, Eduardo Pacheco Rico
Rok vydání: 2022
Předmět:
Zdroj: Purinergic Signal
ISSN: 1573-9546
1573-9538
DOI: 10.1007/s11302-022-09869-z
Popis: Gallic acid (GA) is a secondary metabolite found in plants. It has the ability to cross the blood-brain barrier and, through scavenging properties, has a protective effect in a brain insult model. Alcohol metabolism generates reactive oxygen species (ROS); thus, alcohol abuse has a deleterious effect on the brain. The zebrafish is a vertebrate often used for screening toxic substances and in acute ethanol exposure models. The aim of this study was to evaluate whether GA pretreatment (24 h) prevents the changes induced by acute ethanol exposure (1 h) in the purinergic signaling pathway in the zebrafish brain via degradation of extracellular nucleotides and oxidative stress. The nucleotide cascade promoted by the nucleoside triphosphate diphosphohydrolase (NTPDase) and 5′-nucleotidase was assessed by quantifying nucleotide metabolism. The effect of GA alone at 5 and 10 mg L(−1) did not change the nucleotide levels. Pretreatment with 10 mg L(−1) GA prevented an ethanol-induced increase in ATP and ADP levels. No significant difference was found between the AMP levels of the two pretreatment groups. Pretreatment with 10 mg L(−1) GA prevented ethanol-enhanced lipid peroxidation and dichlorodihydrofluorescein (DCFH) levels. The higher GA concentration was also shown to positively modulate against ethanol-induced effects on superoxide dismutase (SOD), but not on catalase (CAT). This study demonstrated that GA prevents the inhibitory effect of ethanol on NTPDase activity and oxidative stress parameters, thus consequently modulating nucleotide levels that may contribute to the possible protective effects induced by alcohol and purinergic signaling.
Databáze: OpenAIRE
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