Nanoporous Immunoprotective Device for Stem-Cell-Derived β-Cell Replacement Therapy
| Autor: | Audrey Parent, Matthias Hebrok, Holger A. Russ, Tejal A. Desai, Susanna K. Elledge, Qizhi Tang, Jessica L. Allen, Daniel A. Bernards, Gaetano Faleo, Ryan Chang, Karina E. Villanueva |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Biocompatibility Polymers Cell Survival Cell- and Tissue-Based Therapy cell encapsulation device General Physics and Astronomy Bioengineering Regenerative Medicine Article Cell therapy Mice 03 medical and health sciences immunoengineering diabetes Immune system In vivo Nanotechnology Animals General Materials Science Nanoscience & Nanotechnology Cell encapsulation Peritoneal Cavity Transplantation diabetes nanotechnology 5.2 Cellular and gene therapies Teratoma General Engineering Stem Cell Research Embryonic stem cell Nanostructures Cell biology immunoengineering 030104 developmental biology Immunology cell therapy Development of treatments and therapeutic interventions Beta cell Stem cell Stem Cell Transplantation Biotechnology |
| Zdroj: | ACS nano, vol 11, iss 8 |
| ISSN: | 1936-086X 1936-0851 |
| Popis: | Encapsulation of human embryonic stem-cell-differentiated beta cell clusters (hES-βC) holds great promise for cell replacement therapy for the treatment of diabetics without the need for chronic systemic immune suppression. Here, we demonstrate a nanoporous immunoprotective polymer thin film cell encapsulation device that can exclude immune molecules while allowing exchange of oxygen and nutrients necessary for in vitro and in vivo stem cell viability and function. Biocompatibility studies show the device promotes neovascular formation with limited foreign body response in vivo. The device also successfully prevented teratoma escape into the peritoneal cavity of mice. Long-term animal studies demonstrate evidence of engraftment, viability, and function of cells encapsulated in the device after 6 months. Finally, in vivo study confirms that the device was able to effectively immuno-isolate cells from the host immune system. |
| Databáze: | OpenAIRE |
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