Host-specific effect of P1 exchange between two potyviruses
| Autor: | Laurence Quiot, Emilio Yángüez, Pilar Sáenz, Jean Bernard Quiot, Beatriz Salvador, María Otilia Delgadillo, Carmen Simón-Mateo, Juan Antonio García |
|---|---|
| Přispěvatelé: | Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
Molecular Sequence Data Potyvirus Tobacco vein mottling virus Soil Science Plant Science 01 natural sciences Virus Viral Proteins 03 medical and health sciences Chimera (genetics) Tobacco Coding region [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Amino Acid Sequence Molecular Biology Peptide sequence 030304 developmental biology Recombination Genetic 0303 health sciences Sequence Homology Amino Acid biology Potyviridae Original Articles biology.organism_classification Virology Plum Pox Virus Prunus Nicotiana clevelandii Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Molecular Plant Pathology Molecular Plant Pathology, Wiley, 2008, 9 (2), pp.147-155. ⟨10.1111/j.1364-3703.2007.00450.x⟩ |
| ISSN: | 1464-6722 1364-3703 |
| Popis: | International audience; The potyviruses Plum pox virus (PPV) and Tobacco vein mottling virus (TVMV) have distinct host ranges and induce different symptoms in their common herbaceous hosts. To test the relevance of the P1 protein in host compatibility and pathogenicity, hybrid viruses were constructed in which the P1 coding sequence of PPV was completely or partially replaced by the corresponding sequences from TVMV. Infections induced by these chimeric viruses revealed that the TVMV P1 and a PPV/TVMV hybrid P1 proteins are functionally equivalent in herbaceous plants to the P1 protein of a PPV isolate adapted to these hosts, in spite of having high sequence divergence. Moreover, the presence of TVMV P1 sequences enhanced the competence of a low-infectivity PPV-D-derived chimera in Nicotiana clevelandii. Conversely, all PPV/TVMV hybrids were unable to infect Prunus persicae, a specific host for PPV, suggesting that TVMV P1 is not functionally competent in this plant. Together, these data highlight the importance of the P1 protein in defining the virus host range. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text