Toward Liquid Biopsy: Determination of the Humoral Immune Response in Cancer Patients Using HaloTag Fusion Protein-Modified Electrochemical Bioplatforms
| Autor: | Víctor Ruiz-Valdepeñas Montiel, Carmen Poves, José M. Pingarrón, Ana Guzman-Aranguez, Nuria Rodríguez, Rodrigo Barderas, María Garranzo-Asensio, Susana Campuzano, Rebeca M. Torrente-Rodríguez, Luis San Frutos, Gemma Domínguez, Mayte Villalba, María Jesús Fernández-Aceñero |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Models Molecular Colorectal cancer Recombinant Fusion Proteins Biosensing Techniques Analytical Chemistry 03 medical and health sciences Immune system Antigen Limit of Detection Neoplasms medicine Humans Liquid biopsy Autoantibodies Ovarian Neoplasms Chemistry Autoantibody Liquid Biopsy Cancer Electrochemical Techniques Equipment Design medicine.disease Fusion protein Immunity Humoral 030104 developmental biology Immobilized Proteins Cancer research Female Tumor Suppressor Protein p53 Colorectal Neoplasms Biosensor |
| Zdroj: | Analytical chemistry. 88(24) |
| ISSN: | 1520-6882 |
| Popis: | Autoantibodies raised against tumor-associated antigens have shown high promise as clinical biomarkers for reliable diagnosis, prognosis, and therapy monitoring of cancer. An electrochemical disposable biosensor for the specific and sensitive determination of p53-specific autoantibodies has been developed for the first time in this work. This biosensor involves the use of magnetic microcarriers (MBs) modified with covalently immobilized HaloTag fusion p53 protein as solid supports for the selective capture of specific autoantibodies. After magnetic capture of the modified MBs onto screen-printed carbon working electrodes, the amperometric signal using the system hydroquinone/H2O2 was related to the levels of p53-autoantibodies in the sample. The biosensor was applied for the analysis of sera from 24 patients with high-risk of developing colorectal cancer and 6 from patients already diagnosed with colorectal (4) and ovarian (2) cancer. The developed biosensor was able to determine p53 autoantibodies with a... |
| Databáze: | OpenAIRE |
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