Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers
| Autor: | Brandsma, J., Goss, V., Yang, X., Bakke, P. S., Caruso, M., Chanez, P., Dahlen, S. -E., Fowler, S. J., Horvath, I., Krug, N., Montuschi, P., Sanak, M., Sandstrom, T., Shaw, D. E., Chung, K. F., Singer, F., Fleming, L. J., Sousa, A. R., Pandis, I., Bansal, A. T., Sterk, P. J., Djukanovic, R., Postle, A. D., Adcock, I. M., Ahmed, H., Auffray, C., Baribaud, F., Bates, S., Bel, E. H., Bigler, J., Bisgaard, H., Boedigheimer, M. J., Bonnelykke, K., Brinkman, P., Bucchioni, E., Burg, D., Bush, A., Chaiboonchoe, A., Chung, F. K., Compton, C. H., Corfield, J., D'Amico, A., Dahlen, B., De Meulder, B., Erpenbeck, V. J., Erzen, D., Fichtner, K., Fitch, N., Formaggio, E., Frey, U., Gahlemann, M., Geiser, T., Guo, Y., Hashimoto, S., Haughney, J., Hedlin, G., Hekking, P. W., Higenbottam, T., Hohlfeld, J. M., Holweg, C., Howarth, P., James, A. J., Knowles, R. G., Knox, A. J., Lefaudeux, D., Loza, M. J., Lutter, R., Manta, A., Masefield, S., Matthews, J. G., Mazein, A., Meiser, A., Middelveld, R. J. M., Miralpeix, M., Mores, N., Murray, C. S., Musial, J., Myles, D., Pahus, L., Pavlidis, S., Powel, P., Pratico, G., Puig Valls, M., Rao, N., Riley, J., Roberts, A., Roberts, G., Rowe, A., Schofield, J. P. R., Seibold, W., Selby, A., Sigmund, R., Skipp, P. J., Sun, K., Thornton, B., van Aalderen, W. M., van Geest, M., Vestbo, J., Vissing, N. H., Wagener, A. H., Wagers, S. S., Weiszhart, Z., Wheelock, C. E., Wilson, S. J. |
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| Přispěvatelé: | Pulmonology, AII - Inflammatory diseases, Commission of the European Communities, Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Endocrinology Diabetes and Metabolism Clinical Biochemistry Epithelial lining fluid Biochemistry DISEASE Analytical Chemistry Cohort Studies 0302 clinical medicine Fibrosis FIBROSIS Induced sputum Lung COPD Biochemistry and Molecular Biology respiratory system Middle Aged Lipids Healthy Volunteers Cell biology medicine.anatomical_structure Phenotype OBESITY Original Article lipids (amino acids peptides and proteins) Female medicine.symptom Life Sciences & Biomedicine 0301 Analytical Chemistry Bronchoalveolar Lavage Fluid Sputum/chemistry EXPRESSION Adult Settore BIO/14 - FARMACOLOGIA DYNAMIC LIPIDOMICS Adolescent SPUTUM CELL COUNTS BIOMARKERS Biology chronic obstructive pulmonary disease Bronchoalveolar Lavage Fluid/chemistry Endocrinology & Metabolism 03 medical and health sciences Young Adult Lipids/analysis Lung/cytology Lipidomics medicine Humans SURFACTANT LIPIDS Aged Science & Technology pulmonary fibrosis Mass spectrometry Sputum 0601 Biochemistry And Cell Biology 1103 Clinical Sciences Lipid metabolism Pulmonary surfactant medicine.disease Molecular medicine respiratory tract diseases Weight status 030104 developmental biology 030228 respiratory system ASTHMA Biokemi och molekylärbiologi Homeostasis |
| Zdroj: | Metabolomics, 14(10):123. Springer New York Metabolomics U-BIOPRED Study Group 2018, ' Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers ', Metabolomics, vol. 14, no. 10, pp. 123 . https://doi.org/10.1007/s11306-018-1412-2 |
| ISSN: | 1573-3882 |
| DOI: | 10.1007/s11306-018-1412-2 |
| Popis: | Background Lung epithelial lining fluid (ELF)—sampled through sputum induction—is a medium rich in cells, proteins and lipids. However, despite its key role in maintaining lung function, homeostasis and defences, the composition and biology of ELF, especially in respect of lipids, remain incompletely understood. Objectives To characterise the induced sputum lipidome of healthy adult individuals, and to examine associations between different ELF lipid phenotypes and the demographic characteristics within the study cohort. Methods Induced sputum samples were obtained from 41 healthy non-smoking adults, and their lipid compositions analysed using a combination of untargeted shotgun and liquid chromatography mass spectrometry methods. Topological data analysis (TDA) was used to group subjects with comparable sputum lipidomes in order to identify distinct ELF phenotypes. Results The induced sputum lipidome was diverse, comprising a range of different molecular classes, including at least 75 glycerophospholipids, 13 sphingolipids, 5 sterol lipids and 12 neutral glycerolipids. TDA identified two distinct phenotypes differentiated by a higher total lipid content and specific enrichments of diacyl-glycerophosphocholines, -inositols and -glycerols in one group, with enrichments of sterols, glycolipids and sphingolipids in the other. Subjects presenting the lipid-rich ELF phenotype also had significantly higher BMI, but did not differ in respect of other demographic characteristics such as age or gender. Conclusions We provide the first evidence that the ELF lipidome varies significantly between healthy individuals and propose that such differences are related to weight status, highlighting the potential impact of (over)nutrition on lung lipid metabolism. Electronic supplementary material The online version of this article (10.1007/s11306-018-1412-2) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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