Cysteine: an overlooked energy and carbon source

Autor:	Cathrin Kröger, Richard Egelkamp, Marie Charlotte Schoelmerich, Albert Dumnitch, Artur Feld, Thomas Hackl, Johanna Haerdter, Wolfgang R. Streit, Anja Poehlein, Horst Weller, Rolf Daniel, Luise Göbbels
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Biomineralization 0301 basic medicine Magnetic Resonance Spectroscopy Light Acetates Organism chemistry.chemical_classification Carbon Isotopes Multidisciplinary Environmental microbiology biology Bacterial Acetogen Biochemistry Medicine Oxidation-Reduction Intracellular Biotechnology Cadmium Science 030106 microbiology Complex Mixtures Microbiology Article Applied microbiology 03 medical and health sciences Affordable and Clean Energy Moorella thermoacetica Moorella Cysteine Nutrition Bacteria Nanobiotechnology Biological Transport Gene Expression Regulation Bacterial Metabolism biology.organism_classification Carbon 030104 developmental biology Enzyme chemistry Gene Expression Regulation Nanoparticles Energy Metabolism Transcriptome
Zdroj:	Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) Scientific reports, vol 11, iss 1 Scientific Reports
ISSN:	2045-2322
Popis:	Biohybrids composed of microorganisms and nanoparticles have emerged as potential systems for bioenergy and high-value compound production from CO2 and light energy, yet the cellular and metabolic processes within the biological component of this system are still elusive. Here we dissect the biohybrid composed of the anaerobic acetogenic bacterium Moorella thermoacetica and cadmium sulphide nanoparticles (CdS) in terms of physiology, metabolism, enzymatics and transcriptomic profiling. Our analyses show that while the organism does not grow on l-cysteine, it is metabolized to acetate in the biohybrid system and this metabolism is independent of CdS or light. CdS cells have higher metabolic activity, despite an inhibitory effect of Cd2+ on key enzymes, because of an intracellular storage compound linked to arginine metabolism. We identify different routes how cysteine and its oxidized form can be innately metabolized by the model acetogen and what intracellular mechanisms are triggered by cysteine, cadmium or blue light.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f47e2bb8ad5a487d8952967f2552967f https://doaj.org/article/27b8e395ba3447c1b69c821bb1c04bc9 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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