Cysteine: an overlooked energy and carbon source
Autor: | Cathrin Kröger, Richard Egelkamp, Marie Charlotte Schoelmerich, Albert Dumnitch, Artur Feld, Thomas Hackl, Johanna Haerdter, Wolfgang R. Streit, Anja Poehlein, Horst Weller, Rolf Daniel, Luise Göbbels |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Biomineralization
0301 basic medicine Magnetic Resonance Spectroscopy Light Acetates Organism chemistry.chemical_classification Carbon Isotopes Multidisciplinary Environmental microbiology biology Bacterial Acetogen Biochemistry Medicine Oxidation-Reduction Intracellular Biotechnology Cadmium Science 030106 microbiology Complex Mixtures Microbiology Article Applied microbiology 03 medical and health sciences Affordable and Clean Energy Moorella thermoacetica Moorella Cysteine Nutrition Bacteria Nanobiotechnology Biological Transport Gene Expression Regulation Bacterial Metabolism biology.organism_classification Carbon 030104 developmental biology Enzyme chemistry Gene Expression Regulation Nanoparticles Energy Metabolism Transcriptome |
Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) Scientific reports, vol 11, iss 1 Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Biohybrids composed of microorganisms and nanoparticles have emerged as potential systems for bioenergy and high-value compound production from CO2 and light energy, yet the cellular and metabolic processes within the biological component of this system are still elusive. Here we dissect the biohybrid composed of the anaerobic acetogenic bacterium Moorella thermoacetica and cadmium sulphide nanoparticles (CdS) in terms of physiology, metabolism, enzymatics and transcriptomic profiling. Our analyses show that while the organism does not grow on l-cysteine, it is metabolized to acetate in the biohybrid system and this metabolism is independent of CdS or light. CdS cells have higher metabolic activity, despite an inhibitory effect of Cd2+ on key enzymes, because of an intracellular storage compound linked to arginine metabolism. We identify different routes how cysteine and its oxidized form can be innately metabolized by the model acetogen and what intracellular mechanisms are triggered by cysteine, cadmium or blue light. |
Databáze: | OpenAIRE |
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