Molecular basis for the substrate specificity of plant guanine nucleotide exchange factors for ROP
| Autor: | Inka Fricke, Antje Berken |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Rho insert
Rho of plants G protein Protein Conformation Molecular Sequence Data Biophysics RopGEF CDC42 Biology Biochemistry Conserved sequence Substrate Specificity Protein structure Structural Biology DOCK Genetics Guanine Nucleotide Exchange Factors Humans Amino Acid Sequence Molecular Biology Peptide sequence Conserved Sequence Monomeric GTP-Binding Proteins PRONE Arabidopsis Proteins Cell Biology eye diseases Pleckstrin homology domain ROP/RAC Guanine nucleotide exchange factor |
| Zdroj: | FEBS letters. 583(1) |
| ISSN: | 1873-3468 |
| Popis: | Plant G proteins of the ROP/RAC family regulate cellular processes including cytoskeletal rearrangement in polar growth. Activation of the ROP molecular switch is triggered by guanine nucleotide exchange factors. Plant-specific RopGEFs are exclusively active on ROPs despite their high homology to animal Rho proteins. Based on a sequence comparison of ROPs vs. animal Rho proteins together with structural data on distinct ROPs, we identified unique substrate determinants of RopGEF specificity by mutational analysis: asparagine 68 next to switch II, arginine 76 of a putative phosphorylation motif and the Rho insert are essential for substrate recognition by RopGEFs. These data also provide first evidence for a function of the Rho insert in interactions with GEFs. |
| Databáze: | OpenAIRE |
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