Polysaccharide purified from Ganoderma lucidum induces gene expression changes in human dendritic cells and promotes T helper 1 immune response in BALB/c mice
| Autor: | Shiuh-Sheng Lee, Shin-Miao Hou, Yu-Li Lin, Bor-Luen Chiang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Reishi
Ovalbumin medicine.medical_treatment chemical and pharmacologic phenomena Proinflammatory cytokine BALB/c Interferon-gamma Mice Immune system Th2 Cells Adjuvants Immunologic Polysaccharides medicine Animals Humans RNA Messenger Receptors Cytokine Pharmacology CD86 Mice Inbred BALB C CD40 biology Gene Expression Profiling hemic and immune systems Dendritic Cells Th1 Cells biology.organism_classification Molecular biology Interleukin-12 Cytokine Gene chip analysis biology.protein Molecular Medicine Female Receptors Chemokine CD80 Signal Transduction |
| Zdroj: | Molecular pharmacology. 70(2) |
| ISSN: | 0026-895X |
| Popis: | Ganoderma lucidum is a medicinal mushroom in China and other Asian countries. The polysaccharide from G. lucidum (PS-G) is a branched (1--6)-beta-d-glucan moiety. In this study, we examined the effects of PS-G on human monocyte-derived dendritic cells (DCs) with microarray analysis by Human Genome U133 Plus 2.0 GeneChip. In comparing mean signal values between PS-G-treated DCs with untreated DCs, 3477 (17%) probe sets were up-regulated, and 4418 (19%) probe sets were down-regulated after PS-G treatment. These results demonstrate that genes associated with phagocytosis (CD36, CD206, and CD209) are decreased and genes associated with proinflammatory chemokines (CCL20, CCL5, and CCL19), cytokines [interleukin (IL)-27, IL-23A, IL-12A, and IL-12B], and costimulatory molecules (CD40, CD54, CD80, and CD86) are increased. To confirm the microarray data, we further investigated the effect of PS-G on antigen-specific antibody and cytokine production in BALB/c mice. Immunization with ovalbumin (OVA)/PS-G showed that the anti-OVA IgG2a levels were significantly increased compared with OVA alone in BALB/c mice. Together, our data demonstrate that PS-G could effectively promote the activation and maturation of immature DCs, preferring a T helper 1 response. Furthermore, the results also demonstrate that the data from microarray analysis could be correlated with the in vivo effect of the immune-enhancing compound. |
| Databáze: | OpenAIRE |
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