Kallikrein 4 is a potential mediator of cellular interactions between cancer cells and osteoblasts in metastatic prostate cancer
| Autor: | Adrian C. Herington, Rachael Collard, Loan Bui, Judith A. Clements, Jin Gao, David Nicol |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Pathology medicine.medical_specialty Urology Bone Neoplasms Cell Communication urologic and male genital diseases Metastasis Prostate cancer stomatognathic system Cell Movement Cell Line Tumor Bone cell LNCaP Cell Adhesion Humans Medicine RNA Messenger 110300 CLINICAL SCIENCES Osteoblasts business.industry Prostatic Neoplasms Bone metastasis 060106 Cellular Interactions (incl. Adhesion Matrix Cell Wall) Cell migration Osteoblast Prostate-Specific Antigen medicine.disease Coculture Techniques Kallikrein Bone Metastasis Prostate Cancer medicine.anatomical_structure Oncology Cancer cell Cancer research 060107 Enzymes Kallikreins business |
| Zdroj: | Prostate |
| ISSN: | 1097-0045 0270-4137 |
| DOI: | 10.1002/pros.20465 |
| Popis: | BACKGROUND: Prostate cancer (PCa) and bone cell interactions are critical in the metastatic phase. Kallikrein 4 (KLK4/hK4) is expressed in both PCa and mineralized tissues. We determined if KLK4/hK4 expression was associated with, and influenced by, the bone environment of metastatic PCa. METHODS: Immunohistochemistry, in vitro co-culture, cell migration, and attachment assays. RESULTS: hK4 was localized to tumor cells and osteoblasts in bone metastases. KLK4/hK4 increased in LNCaP and PC3 cells co-cultured with SaOs2 cells; SaOs2 KLK4/hK4 was unchanged. Co-culture did not affect cell proliferation but altered alkaline phosphatase activity/mRNA levels in SaOs2 cells. KLK4-transfected PC3 cells had increased migration towards SaOs2 conditioned medium and greater attachment to the bone-matrix proteins, collagens I and IV. CONCLUSIONS: hK4 expression and interaction with both tumor cells and osteoblasts suggests a role for hK4 in PCa bone metastasis. Whether this observation is unique to bone metastasis or reflects a role for hK4 in PCa metastasis generally is yet to be established. |
| Databáze: | OpenAIRE |
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