Protective and therapeutic effect of hydrogen sulfide on hemorrhagic cystitis and testis dysfunction induced with cyclophosphamide
Autor: | Neziha Senem Ari, Semra Yiğitaslan, Yasemin Teksen, Orhan Özatik, Fikriye Yasemin Özatik |
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Rok vydání: | 2020 |
Předmět: |
Male
medicine.medical_specialty Antioxidant Cyclophosphamide medicine.medical_treatment hydrogen sulfide Sodium hydrosulfide Hemorrhage testis Testicular Diseases Article Male infertility chemistry.chemical_compound Internal medicine Cystitis medicine Animals Testosterone bladder business.industry Interleukin-6 Therapeutic effect Acrolein General Medicine medicine.disease Interleukin-10 Rats Endocrinology chemistry Follicle Stimulating Hormone business medicine.drug Hemorrhagic cystitis |
Zdroj: | Turkish Journal of Medical Sciences |
ISSN: | 1303-6165 |
Popis: | Backround/aim Cyclophosphamide (CP) is a drug used for treatment of many malignant diseases. However, it can cause serious side effects such as hemorrhagic cystitis and male infertility. Hydrogen sulfide (H2S) is a gaseous mediator and is suggested to have antioxidant, antiinflammatory, and antiapoptotic effects. In this study, dose-dependent effects of H2S donor sodium hydrosulfide (NaHS) on cyclophosphamide-induced hemorrhagic cystitis and testicular dysfunction were investigated in rats. Material and methods Rats were divided into 5 groups (n = 8): control, CP, NaHS25 μmol/kg, NaHS50 μmol/kg, and NaHS100 μmol/ kg. After treatment for 7 days intraperitoneally (ip), a single ip dose of CP 200 mg/kg was given on the 8th day. Then, treatment was continued for 7 days. In bladder and testicular tissues, IL 6, IL 10, cGMP, NO, H2S, FSH, LH, and testosterone levels were measured by ELISA. Histopathological examination with H&E staining, as well as immunohistochemical staining for acrolein in bladder and caspase-3 and APAF-1 in testis were performed. Results NaHS prevented the increased IL 6 and IL 10 values induced by CP as well as prevented the decrease in cGMP values associated with CP. There was no significant change in FSH values, but the LH value, which increased with CP, decreased with 25, 50, and 100 μmol/kg NaHS. In contrast, testosterone decreased in the CP group and increased in the treatment groups. NaHS was effective in many biochemical and histopathological parameters at 25 and 50 μmol/kg doses, and this effect decreased at 100 μmol/kg dose. Conclusion H2S has a protective and therapeutic effect on hemorrhagic cystitis and testicular dysfunction induced by cyclophosphamide. It can be suggested that H2S is a promising molecule in facilitating cancer treatment. |
Databáze: | OpenAIRE |
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