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PDF file - 255K, Effective inhibition of in vitro tumor cell migration by EMD 1214063 and EMD 1204831. A classical "wound healing" test was performed to assess the impact of EMD 1214063 (A) and EMD 1204831 (B) on tumor cell migration. In brief, H441 lung cancer cells were seeded on day 1 (3 x 105 cells/well in 24-well plate), serum-starved on day 2, and treated on day 3 with the indicated concentrations of the EMD compounds for 1 h. After "scratching" the cell layer, cells were incubated in serum-free medium containing 100 ng/mL HGF and the indicated concentrations of the compounds for 24h. Controls were untreated cells with (left) or without HGF (right). At 0 h and 24 h, cells were washed, fixed with ice-cold methanol, and stained with crystal violet (0.05% in 50% ethanol). Treatment with EMD 1214063 or EMD 1204831 resulted in a strong inhibition of tumor cell migration, preventing wound healing. Pictures were taken at 5x magnification. Data are representative of two independent experiments. |
