Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats

Autor: Álvaro de Oliveira Franco, Roberto Farina de Almeida, Pedro Espitia-Pérez, Diogo P. Moraes, Moara Rodrigues Mingori, José Cláudio Fonseca Moreira, Alexsander Alves Teixeira, José F. Torres-Ávila, Victória Schmidtt, Alana Castro Panzenhagen, Carlos Eduardo Schnorr, Paolla Rissi Silva Hermann, Helen Tais da Rosa-Silva
Rok vydání: 2019
Předmět:
Male
Health
Toxicology and Mutagenesis
0208 environmental biotechnology
02 engineering and technology
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Nervous System
chemistry.chemical_compound
Double exposure
Pregnancy
Methylmercury
Liver injury
biology
General Medicine
Methylmercury Compounds
Pollution
Breast Feeding
Liver
Environmental Pollutants
Female
Oxidation-Reduction
Locomotion
Signal Transduction
medicine.medical_specialty
Environmental Engineering
Fetus
Internal medicine
medicine
Neurotoxicity
Environmental Chemistry
Animals
Humans
Rats
Wistar
Protein kinase B
PI3K/AKT/mTOR pathway
0105 earth and related environmental sciences
business.industry
Hepatotoxicity
Public Health
Environmental and Occupational Health
General Chemistry
South America
medicine.disease
020801 environmental engineering
Rats
Endocrinology
chemistry
biology.protein
NeuN
business
Oxidative stress
Zdroj: Chemosphere
Repositorio Digital USB
Universidad Simón Bolívar
instacron:Universidad Simón Bolívar
ISSN: 1879-1298
DOI: 10.1016/j.chemosphere.2019.125400
Popis: Methylmercury (MeHg) is an organic bioaccumulated mercury derivative that strongly affects the environment and represents a public health problem primarily to riparian communities in South America. Our objective was to investigate the hepatic and neurological effects of MeHg exposure during the phases foetal and breast-feeding and adult in Wistar rats. Wistar rats (n = 10) were divided into 3 groups. Control group received mineral oil; The simple exposure (SE) group was exposed only in adulthood (0.5 mg/kg/day); and double exposure (DE) was pre-exposed to MeHg 0.5 mg/kg/day during pregnancy and breastfeeding (±40 days) and re-exposed to MeHg for 45 days from day 100. After, we evaluated possible abnormalities. Behavioral and biochemical parameters in liver and occipital cortex (CO), markers of liver injury, redox and AKT/GSK3β/mTOR signaling pathway. Our results showed that both groups treated with MeHg presented significant alterations, such as decreased locomotion and exploration and impaired visuospatial perception. The rats exposed to MeHg showed severe liver damage and increased hepatic glycogen concentration. The MeHg groups showed significant impairment in redox balance and oxidative damage to liver macromolecules and CO. MeHg upregulated the AKT/GSK3β/mTOR pathway and the phosphorylated form of the Tau protein. In addition, we found a reduction in NeuN and GFAP immunocontent. These results represent the first approach to the hepatotoxic and neural effects of foetal and adult MeHg exposure.
Databáze: OpenAIRE
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