Effects of low-levels of three hexabromocyclododecane diastereomers on the metabolic profiles of pak choi leaves using high-throughput untargeted metabolomics approach
Autor: | Yanwei Zhang, Tan Dongfei, Zeying He, Yuehua Wang, Qiqi Guo, Xiaowei Liu |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Antioxidant Health Toxicology and Mutagenesis medicine.medical_treatment 010501 environmental sciences Secondary metabolite Toxicology 01 natural sciences Antioxidants Mice 03 medical and health sciences chemistry.chemical_compound Metabolomics medicine Animals Humans Soil Pollutants Amino Acids 0105 earth and related environmental sciences Hexabromocyclododecane chemistry.chemical_classification Chemistry Brassica rapa Fishes General Medicine Carbohydrate Pollution Hydrocarbons Brominated Amino acid Metabolic pathway 030104 developmental biology Biochemistry Toxicity Metabolome Metabolic Networks and Pathways medicine.drug |
Zdroj: | Environmental Pollution. 242:1961-1969 |
ISSN: | 0269-7491 |
DOI: | 10.1016/j.envpol.2018.07.062 |
Popis: | The ecological toxicity of hexabromocyclododecane (HBCD) on animals, including fish and mice, has been reported, but its effects in plants, particularly its toxic mechanism, have rarely been investigated. An untargeted metabolomics approach for comprehensive assessment was selected to study the alterations in the metabolic profiles in pak choi leaves induced by exposure to trace-level amounts of HBCD diastereomers over 30 days. A supervised orthogonal partial least-squares-discriminant analysis (OPLS-DA) was performed to investigate differences between the HBCD and control groups. The discriminating metabolites were identified using public databases. The results indicated that the toxicity of the HBCD diastereomers was ordered as γ-HBCD > α-HBCD > β-HBCD. 13 metabolites were identified as potential biomarkers to discriminate the presence of HBCD toxicity. The lipid, carbohydrate, nucleotide and amino acid metabolic pathways affected were found in accordance with animals and humans, and also HBCD could induce the interference of the secondary metabolite pathways. The system of the stress defences was activated, including signalling pathway, antioxidant defence system, shikimate and phenylpropanoid metabolism. The carbohydrate and amino acid metabolism were disturbed by HBCD intervention, and the lipid, amino acid and secondary metabolite metabolism were regulated for HBCD stress prevention. These results provide insights into the mechanism and degree of HBCD phytotoxicity. |
Databáze: | OpenAIRE |
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