Using environmental DNA methods to survey for rare groundwater fauna: Detection of an endangered endemic cave crayfish in northern Alabama

Autor:	Matthew L. Niemiller, Spencer H. Boyd, K. Denise Kendall Niemiller, Katherine E. Dooley, Jennifer Nix
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Topography Fauna Endangered species Biodiversity Gene Expression Astacoidea Polymerase Chain Reaction Geographical locations Environmental DNA Groundwater Conservation Science Multidisciplinary geography.geographical_feature_category biology Ecology Eukaryota Crustaceans Spring Caves Alabama Medicine Seasons Research Article Conservation of Natural Resources Arthropoda Science Arthropod Proteins Electron Transport Complex IV Cave Surface Water Animals geography Landforms Ecology and Environmental Sciences Endangered Species Organisms Biology and Life Sciences Geomorphology Karst biology.organism_classification Karst Features Invertebrates Crayfish DNA Environmental United States Threatened species North America Earth Sciences Hydrology People and places Cambarus Zoology Animal Distribution
Zdroj:	PLoS ONE, Vol 15, Iss 12, p e0242741 (2020) PLoS ONE
ISSN:	1932-6203
Popis:	The conservation and management of subterranean biodiversity is hindered by a lack of knowledge on the true distributions for many species, e.g., the Wallacean shortfall. In recent years, several studies have demonstrated the potential of environmental DNA (eDNA) as an effective approach to detect and monitor biodiversity, including rare, threatened, and endangered taxa. However, there are few eDNA studies of groundwater fauna. Here we report the results of the development and implementation of an eDNA assay targeting a short fragment of the mitochondrial CO1 locus of a critically imperiled cave crayfish, the Sweet Home Alabama Cave Crayfish (Cambarus speleocoopi), known from just four cave systems in the Interior Plateau karst region of northern Alabama. We detected C. speleocoopi DNA from water samples collected at 5 of 16 sites sampled (caves and springs), including two historical sites as well as three additional and potentially new sites in Marshall County, Alabama. All three of these sites were within 2 km of historical sites. Our study is the first to detect a groundwater crustacean in the Interior Plateau karst region. Additionally, our study contributes to the growing literature that eDNA is a viable complementary tool for detection and monitoring of a fauna that is difficult to survey and study using traditional approaches.
Databáze:	OpenAIRE
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