Are globoseries glycosphingolipids SSEA-3 and -4 markers for stem cells derived from human umbilical cord blood?
| Autor: | Anita Laitinen, Tero Satomaa, Leena Valmu, Jari Natunen, Saara Laitinen, Halina Miller-Podraza, Heidi Anderson, Annamari Heiskanen, Tia Hirvonen, Suvi Natunen, Virve Pitkänen, Heli Suila |
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| Rok vydání: | 2010 |
| Předmět: |
Stage-Specific Embryonic Antigens
Gene Expression Biology Stem cell marker Glycosphingolipids Genetics Humans Antigens Tumor-Associated Carbohydrate Molecular Biology Cells Cultured Stem cell transplantation for articular cartilage repair Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology General Medicine Fetal Blood Flow Cytometry Hematopoietic Stem Cells Embryonic stem cell Molecular biology Haematopoiesis Cord blood embryonic structures Stem cell Adult stem cell |
| Zdroj: | Journal of molecular cell biology. 3(2) |
| ISSN: | 1759-4685 |
| Popis: | Umbilical cord blood (UCB) is an efficient and valuable source of hematopoietic stem cells (HSCs) for transplantation. In addition to HSCs it harbours low amounts of mesenchymal stem cells (MSCs). No single marker to identify cord blood-derived stem cells, or to indicate their multipotent phenotype, has been characterized so far. SSEA-3 and -4 are cell surface globoseries glycosphingolipid epitopes that are commonly used as markers for human embryonic stem cells, where SSEA-3 rapidly disappears when the cells start to differentiate. Lately SSEA-3 and -4 have also been observed in MSCs. As there is an ongoing discussion and variation of stem-cell markers between laboratories, we have now comprehensively characterized the expression of these epitopes in both the multipotent stem-cell types derived from UCB. We have performed complementary analysis using gene expression analysis, mass spectrometry and immunochemical methods, including both flow cytometry and immunofluoresence microscopy. SSEA-4, but not SSEA-3, was expressed on MSCs but absent from HSCs. Our findings indicate that SSEA-3 and/or -4 may not be optimal markers for multipotency in the case of stem cells derived from cord blood, as their expression may be altered by cell-culture conditions. |
| Databáze: | OpenAIRE |
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