Kinin B1 receptor gene ablation affects hypothalamic CART production
| Autor: | Thiago Vignoli, João Bosco Pesquero, Carolina Batista, Vicencia Sales, Rita Sinigaglia-Coimbra, Fabiana Louise Motta, Hugo A. M. Torres, Luiz E. Mello, Vânia D'Almeida, Michael Bader, Joelcimar M. da Silva, Jackson C. Bittencourt, Gabriela Filoso Barnabe, Francine O. Goeldner |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Cart medicine.medical_specialty medicine.medical_treatment Clinical Biochemistry Adipose tissue Nerve Tissue Proteins Kinins Biology Biochemistry Energy homeostasis Mice Internal medicine medicine Animals Neuropeptide Y Obesity RNA Messenger Receptor Molecular Biology In Situ Hybridization Mice Knockout Leptin Insulin Body Weight Kinin ANATOMIA Immunohistochemistry Mice Inbred C57BL Endocrinology Hypothalamic Area Lateral Knockout mouse Energy Intake |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | A role for the kinin B1 receptor in energy-homeostatic processes was implicated in previous studies; notably, the studies where kinin B1 receptor knockout mice (B1-/-) were shown to have impaired adiposity, impaired leptin and insulin production, lower feed efficiency, protection from liver steatosis and diet-induced obesity when fed a high fat diet (HFD). In particular, in a model where the B1 receptor is expressed exclusively in the adipose tissue, it rescues the plasma insulin concentration and the weight gain seen in wild type mice. Taking into consideration that leptin participates in the formation of hypothalamic nuclei, which modulate energy expenditure, and feeding behavior, we hypothesized that these brain regions could also be altered in B1-/- mice. We observed for the first time a difference in the gene expression pattern of cocaine and amphetamine related transcript (CART) in the (lateral hypothalamic area (LHA) resulting from the deletion of the kinin B1 receptor gene. The correlation between CART expression in the LHA and the thwarting of diet-induced obesity corroborates independent correlations between CART and obesity. Furthermore, it seems to indicate that the mechanism underlying the ‘lean’ phenotype of B1-/- mice does not stem solely from changes in peripheral tissues but may also receive contributions from changes in the hypothalamic machinery involved in energy homeostasis processes. |
| Databáze: | OpenAIRE |
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