Diverse Mesorhizobium bacteria nodulate native Astragalus and Oxytropis in arctic and subarctic areas in Eurasia
| Autor: | Kristina Lindström, Osei Yaw Ampomah, Kerstin Huss-Danell, Seyed Abdollah Mousavi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial N-Acetylglucosaminyltransferases Applied Microbiology and Biotechnology Microbiology DNA Ribosomal Plant Root Nodulation Oxytropis Rhizobia Russia 03 medical and health sciences Bacterial Proteins RNA Ribosomal 16S Botany Cluster Analysis Ecology Evolution Behavior and Systematics Phylogeny Astragalus alpinus Sweden Phylogenetic tree biology Arctic Regions Mesorhizobium food and beverages Genetic Variation Astragalus Plant DNA-Directed RNA Polymerases 16S ribosomal RNA rpoB biology.organism_classification Housekeeping gene Rec A Recombinases 030104 developmental biology Oxidoreductases Root Nodules Plant Multilocus Sequence Typing |
| Zdroj: | Systematic and applied microbiology. 40(1) |
| ISSN: | 1618-0984 |
| Popis: | Rhizobia nodulating native Astragalus and Oxytropis spp. in Northern Europe are not well-studied. In this study, we isolated bacteria from nodules of four Astragalus spp. and two Oxytropis spp. from the arctic and subarctic regions of Sweden and Russia. The phylogenetic analyses were performed by using sequences of three housekeeping genes (16S rRNA, rpoB and recA) and two accessory genes (nodC and nifH). The results of our multilocus sequence analysis (MLSA) of the three housekeeping genes tree showed that all the 13 isolates belonged to the genus Mesorhizobium and were positioned in six clades. Our concatenated housekeeping gene tree also suggested that the isolates nodulating Astragalus inopinatus, Astragalus frigidus, Astragalus alpinus ssp. alpinus and Oxytropis revoluta might be designated as four new Mesorhizobium species. The 13 isolates were grouped in three clades in the nodC and nifH trees. 15N analysis suggested that the legumes in association with these isolates were actively fixing nitrogen. |
| Databáze: | OpenAIRE |
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