Caspase activity in post mortem muscle and its relation to cattle handling practices
| Autor: | Daniel Franco, Noelia Aldai, Enrique Sentandreu, Claudia Fuente-García, Mamen Oliván, Miguel Angel Sentandreu, S. García-Torres, Luis Javier R. Barron |
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| Přispěvatelé: | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España), Ministerio de Ciencia, Innovación y Universidades (España), European Commission, CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Meat packing industry
030309 nutrition & dietetics Apoptosis Rubia Gallega meat quality oxidative stress longissimus Animal Husbandry Caspase Caspase activity 0303 health sciences mechanisms Nutrition and Dietetics biology apoptosis 04 agricultural and veterinary sciences 040401 food science beef Breed cell death caspases Caspases Biotechnology Cell death Programmed cell death Meat Andrology 03 medical and health sciences Animal management 0404 agricultural biotechnology meat tenderness Animals yak meat Metabolic Stress Meat quality Muscle Skeletal business.industry biomarkers biology.organism_classification pre-slaughter Bos taurus Postmortem Changes animal management biology.protein Cattle activation business Agronomy and Crop Science Food Science |
| Zdroj: | Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Addi. Archivo Digital para la Docencia y la Investigación instname Digital.CSIC. Repositorio Institucional del CSIC |
| Popis: | BACKGROUND: Animal handling practices are one of the factors majorly affecting animal metabolism prior to slaughter. This phenomenon increases the occurrence of meat quality defects such as dark cutting-beef, causing high economical losses in the meat industry. Under this framework, the assessment of apoptosis onset in post mortem muscle was proposed as a novel approach to reveal biochemical characteristics in several Spanish bovine breeds (Asturiana de los Valles, Retinta and Rubia Gallega) managed under different production systems (intensive versus semi-extensive) and transport/lairage conditions (mixing versus not mixing with unfamiliar animals). To do so, the activities of initiator caspase 9 and executioner caspases 3/7 were determined in Longissimus thoracis et lumborum muscle at three early post mortem times (2, 8, and 24 h). RESULTS: Breed effect and transport/lairage conditions were the most relevant factors that influenced both caspase activities over post mortem time, showing Rubia Gallega breed a completely different behavior compared to Asturiana de los Valles and Retinta breeds. Moreover, it is postulated that apoptosis cascade is initiated via the activation of caspase 9 under hypoxic or metabolic stress followed by the activation of executioner caspases 3/7. CONCLUSIONS: Assessment of apoptosis on post mortem muscle can be a novel approach to study the influence of animal handling on muscle metabolism and post mortem cell death and its consequences on meat quality traits. Claudia Fuente-Garcia thanks the Department of Economic Development & Infrastructures of the Basque Government for the doctoral fellowship. This work was funded by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (RTA2014-00034-C04), Agencia Estatal de Investigación (RTI2018-096162-R-C22) and FEDER funds. |
| Databáze: | OpenAIRE |
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