The Effect of Perfluorocarbon-Based Artificial Oxygen Carriers on Tissue-Engineered Trachea
| Autor: | Monika Hilbe, Clemens van Blitterswijk, Rolf Jaussi, Sven Hillinger, Donat R. Spahn, Claudio Contaldo, Ashraf Mohammad El-Badry, Gustavo A. Higuera, Manfred Welti, Rudolf Steiner, Walter Weder, Qiang Tan, Qingquan Luo |
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| Přispěvatelé: | University of Zurich, Tan, Q |
| Rok vydání: | 2009 |
| Předmět: |
Microdialysis
1303 Biochemistry 10255 Clinic for Thoracic Surgery 10216 Institute of Anesthesiology Partial Pressure Polyesters Polyurethanes Sus scrofa Biomedical Engineering 10184 Institute of Veterinary Pathology 2204 Biomedical Engineering 610 Medicine & health Bioengineering Biochemistry Chorioallantoic Membrane Epithelium Biomaterials Glycosaminoglycan Chondrocytes medicine Animals Humans 10266 Clinic for Reconstructive Surgery Glycosaminoglycans Fluorocarbons 1502 Bioengineering Tissue Engineering Tissue Scaffolds Foreign-Body Reaction 2502 Biomaterials Histology Dermis Metabolism Molecular biology Hydrocarbons Brominated Rats Oxygen Trachea Chorioallantoic membrane 10022 Division of Surgical Research medicine.anatomical_structure Oxygent 570 Life sciences biology Perfusion |
| Zdroj: | Tissue Engineering Part A. 15:2471-2480 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2008.0461 |
| Popis: | The biological effect of the perfluorocarbon-based artificial oxygen carrier (Oxygent) was investigated in tissue-engineered trachea (TET) construction. Media supplemented with and without 10% Oxygent were compared in all assessments. Partial tissue oxygen tension (PtO(2)) was measured with polarographic microprobes; epithelial metabolism was monitored by microdialysis inside the TET epithelium perfused with the medium underneath. Chondrocyte-DegraPol constructs were cultured for 1 month with the medium before glycosaminoglycan assessment and histology. Tissue reaction of TET epithelial scaffolds immersed with the medium was evaluated on the chick embryo chorioallantoic membrane. Oxygent perfusion medium increased the TET epithelial PtO(2) (51.2 +/- 0.3 mm Hg vs. 33.4 +/- 0.3 mm Hg at 200 microm thickness; 12.5 +/- 0.1 mm Hg vs. 3.1 +/- 0.1 mm Hg at 400 microm thickness, p < 0.01) and decreased the lactate concentration (0.63 +/- 0.08 vs. 0.80 +/- 0.06 mmol/L, p < 0.05), lactate/pyruvate (1.87 +/- 0.26 vs. 3.36 +/- 10.13, p < 0.05), and lactate/glucose ratios (0.10 +/- 0.00 vs. 0.29 +/- 0.14, p < 0.05). Chondrocyte-DegraPol in Oxygent group presented lower glycosaminoglycan value (0.03 +/- 0.00 vs. 0.13 +/- 0.00, p < 0.05); histology slides showed poor acid mucopolysaccharides formation. Orthogonal polarization spectral imaging showed no difference in functional capillary density between the scaffolds cultured on chorioallantoic membranes. The foreign body reaction was similar in both groups. We conclude that Oxygent increases TET epithelial PtO(2), improves epithelial metabolism, does not impair angiogenesis, and tends to slow cartilage tissue formation. |
| Databáze: | OpenAIRE |
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