Mutation, DNA labeling, and transformation of BHK-21/CL 13 cells by MNNG, and nitrosocimetidine
| Autor: | Louis R. Barrows, Charles T. Gombar, Peter N. Magee |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Methylnitronitrosoguanidine
Guanine Cell Survival viruses Mutagen Kidney Toxicology medicine.disease_cause Guanidines Cell Line chemistry.chemical_compound Cricetinae Genetics medicine Baby hamster kidney cell Animals Mutation Mesocricetus biology Mutagenicity Tests urogenital system Cell growth DNA biology.organism_classification Molecular biology Transformation (genetics) Cell Transformation Neoplastic chemistry Cell culture Cimetidine Mutagens |
| Zdroj: | Mutation Research/Genetic Toxicology. 102:145-158 |
| ISSN: | 0165-1218 |
| Popis: | N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) has been reported to induce BHK-21/Cl 13 cell growth in agar suspension. To determine if MNNG was also mutagenic to BHK cells, an ouabain-resistance mutation assay was established using these cells. In this system MNNG was compared to nitrosocimetidine (NC). MNNG and NC did induce ouabain-resistant mutations in BHK cells. The ability of the test compounds to methylate DNA in BHK cells was also determined, and both MNNG and NC yielded detectable levels of 7-methylguanine in treated cells. MNNG and NC were tested for the ability to transform BHK cells, and did. NC was found to be as effective a mutagen and transforming agent in BHK cells as MNNG when administered at equitoxic concentrations; approx. 4-fold less effective at equimolar concentrations. |
| Databáze: | OpenAIRE |
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