3,5-T2 and 3,3′,5-T3 Regulate Cerebellar Thyroid Hormone Signalling and Myelin Molecular Dynamics in Tilapia
| Autor: | Maricela Luna, Y. Hernández-Linares, Patricia Kurczyn Villalobos, Aurea Orozco, Aurora Olvera, C. Lozano-Flores, Alfredo Varela-Echavarría |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Cerebellum Molecular biology Diiodothyronines Cell Culture Techniques Thyroid Gland lcsh:Medicine DIO2 Granular layer Biology Article 03 medical and health sciences Myelin 0302 clinical medicine Developmental biology medicine Animals Progenitor cell lcsh:Science Myelin Sheath Multidisciplinary lcsh:R Thyroid Cichlids Cell biology 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation nervous system Models Animal Triiodothyronine lcsh:Q 030217 neurology & neurosurgery Ex vivo Neuroscience Signal Transduction Hormone |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In contrast to mammalian adults, myelination in teleosts occurs throughout their lifespan and most of the progenitor cells are originated in the cerebellum. To understand the role that thyroid hormones (THs) play in juvenile cerebellar myelination in teleosts, we identified and localised the expression of genes involved in TH signalling (mct8, oatp1c1, dio2, dio3, thraa and l-thrb1) and analysed the effects of the two bioactive THs, T2 and T3, upon their regulation, as well as upon some structural components of the myelination process. Ex vivo approaches using organotypic cerebellar cultures followed by FISH and qPCR showed gene-specific localisation and regulation of TH signalling genes in the cerebellar nuclei. In vivo approaches using methimazole (MMI)-treated juvenile tilapias replaced with low doses of T3 and T2 showed by immunofluorescence that myelin fibres in the cerebellum are more abundant in the granular layer and that their visible size is reduced after MMI treatment but partially restored with TH replacement, suggesting that low doses of TH promote the re-myelination process in an altered condition. Together, our data support the idea that T2 and T3 promote myelination via different pathways and prompt T2 as a target for further analysis as a promising therapy for hypomyelination. |
| Databáze: | OpenAIRE |
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