Multimodal molecular imaging of integrin αvβ3 for in vivo detection of pancreatic cancer
| Autor: | Jens T. Siveke, Ambros J. Beer, Vasilis Ntziachristos, Irene Esposito, Neal C. Burton, Karolin Pohle, George Themelis, Katja Steiger, Hans-Jürgen Wester, Athanasios Sarantopoulos, Christoph W. Michalski, Pouyan Mohajerani, Rickmer Braren, Jörg Kleeff, Stefan Stangl, E Kalideris, Xiaopeng Ma, Roland M. Schmid, Marija Trajkovic-Arsic |
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| Rok vydání: | 2014 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Pathology medicine.medical_specialty endocrine system diseases Adenocarcinoma medicine.disease_cause Multimodal Imaging Intraoperative Period Mice In vivo Pancreatic cancer Cell Line Tumor Medicine Animals Humans Radiology Nuclear Medicine and imaging Tomography Fluorescence Molecular Tomography Genetically Engineered Mice Integrin αvβ3 Pancreatic Cancer Positron Emission Tomography medicine.diagnostic_test business.industry Optical Imaging medicine.disease Integrin alphaVbeta3 digestive system diseases Gene Expression Regulation Neoplastic Pancreatic Neoplasms Positron emission tomography Positron-Emission Tomography Immunohistochemistry Feasibility Studies Female KRAS Molecular imaging business Preclinical imaging |
| Zdroj: | J. Nucl. Med. 55, 446-451 (2014) |
| ISSN: | 1535-5667 |
| Popis: | UNLABELLED: Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease. Late detection of then nonresectable or metastasized tumors emphasizes the need for novel imaging approaches. Here, we report on so far nonexploited potentials of αvβ3 integrin-targeted molecular imaging technologies for detection of PDAC using genetically engineered mouse models. METHODS: Immunohistochemistry and Western blot were used for characterization of αvβ3 expression in murine and human PDAC. We applied IntegriSense 680 fluorescence molecular tomography, intraoperative fluorescence imaging, and (68)Ga-NODAGA-RGD PET for αvβ3 integrin molecular in vivo imaging of spontaneous PDAC occurring in Ptf1a(+/Cre);Kras(+/LSL-G12D);p53(LoxP/LoxP) mice. (NODAGA is 1,4,7-triazacyclononane-1,4-bis[acetic acid]-7-[2-glutaric acid] and RGD is arginine-glycine-aspartic acid.) RESULTS: αvβ3 integrin is expressed in tumor cells of human and murine PDAC. IntegriSense fluorescence molecular tomography and (68)Ga-NODAGA-RGD PET enabled faithful visualization of PDAC. Furthermore, intraoperative optical imaging with IntegriSense 680 allowed good delineation of tumor borders. CONCLUSION: Imaging approaches targeting αvβ3 integrin expand the potential of molecular imaging for identification of αvβ3-positive PDAC with potential implications in early detection, fluorescence-guided surgery, and therapy monitoring. |
| Databáze: | OpenAIRE |
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