Bone Strength/Bone Mass Discrepancy in Glucocorticoid‐Treated Adult Mice

Autor: Nancy E Lane, Donald B. Kimmel, Jeffrey S. Nyman, Sasidhar Uppuganti, Kenneth J. Chmiel, Alanna M. Dubrovsky
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: JBMR Plus, Vol 5, Iss 3, Pp n/a-n/a (2021)
JBMR Plus
ISSN: 2473-4039
Popis: Glucocorticoids increase bone fragility in patients in a manner that is underestimated by bone mass measurement. This study aimed to determine if the adult mouse could model this bone strength/bone mass discrepancy. Forty‐two 13‐week‐old BALB/cJ mice were randomized into vehicle and glucocorticoid groups, implanted with vehicle or 6‐methylprednisolone pellets, and necropsied after 60 and 120 days. Bone strength and bone mass/microarchitecture were assessed at the right central femur (CF; cortical‐bone–rich) and sixth lumbar vertebral body (LVB6; trabecular‐bone–rich). Bound water (BW) of the whole right femur was analyzed by proton‐nuclear magnetic resonance (1H‐NMR) relaxometry. Data were analyzed by two‐factor ANOVA with time (day 60 and day 120) and treatment (vehicle and glucocorticoid) as main effects for all data. Significant interactions were further analyzed with a Tukey's post hoc test. Most bone strength measures in the CF were lower in the glucocorticoid group, regardless of the duration of treatment, with no time × treatment interaction. However, bone mass measures in the CF showed a significant time × treatment interaction (p = 0.0001). Bone strength measures in LVB6 showed a time × treatment interaction (p
Databáze: OpenAIRE
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