Comparison of the nine polymorphic membrane proteins of Chlamydia trachomatis for their ability to induce protective immune responses in mice against a C. muridarum challenge
| Autor: | Alison Favaroni, Sören E.T. Luczak, Philipp T. Hanisch, Sukumar Pal, Johannes H. Hegemann, Delia F. Tifrea, Luis M. de la Maza |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Serotype Chlamydia muridarum Cross Protection 030106 microbiology Chlamydia trachomatis Biology Cross Reactions medicine.disease_cause Article Microbiology law.invention 03 medical and health sciences Immune system Antigen Adjuvants Immunologic law medicine Animals Humans Lung Mice Inbred BALB C Vaccines Synthetic Chlamydia General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health Membrane Proteins Chlamydia Infections medicine.disease Vaccination Disease Models Animal 030104 developmental biology Infectious Diseases Treatment Outcome Immunology Bacterial Vaccines Vaccines Subunit Recombinant DNA Molecular Medicine Nasal administration Bacterial Outer Membrane Proteins |
| Zdroj: | Vaccine. 35(19) |
| ISSN: | 1873-2518 |
| Popis: | Objectives To test vaccines, formulated with novel antigens, to protect mice against Chlamydia infections. Methods To determine the ability of polymorphic membrane proteins (Pmps) to induce cross-species protective immune responses, recombinant fragments from all nine C. trachomatis serovar E Pmps were used to vaccinate BALB/c mice utilizing CpG-1826 and Montanide ISA 720 as adjuvants. C. muridarum recombinant MOMP and PBS, formulated with the same adjuvants, were used as positive and negative controls, respectively. Mice were challenged intranasally with 104 inclusion-forming units (IFU) of C. muridarum. Animals were weighed daily and at 10 days post-challenge, they were euthanized, their lungs harvested, weighed and the number of chlamydial IFU counted. Results Following vaccination the nine Pmps elicited immune responses. Based on body weight changes, or number of IFU recovered from lungs, mice vaccinated with Pmp C, G or H were the best protected. For example, over the 10-day period, the negative control group vaccinated with PBS lost significantly more body weight than mice immunized with PmpC or G (P |
| Databáze: | OpenAIRE |
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