Comparison of optimal performance at 300keV of three direct electron detectors for use in low dose electron microscopy
| Autor: | Greg McMullan, A.R. Faruqi, Daniel K. Clare, Richard Henderson |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Physics - Instrumentation and Detectors
Materials science FOS: Physical sciences 02 engineering and technology Electron DQE Radiation bcs Article law.invention Detective quantum efficiency 03 medical and health sciences Optics law Optical transfer function Instrumentation 030304 developmental biology 0303 health sciences business.industry CMOS Detector Instrumentation and Detectors (physics.ins-det) 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials MTF Spatial frequency Electron microscope 0210 nano-technology business |
| Zdroj: | Ultramicroscopy |
| ISSN: | 0304-3991 |
| DOI: | 10.1016/j.ultramic.2014.08.002 |
| Popis: | Low dose electron imaging applications such as electron cryo-microscopy are now benefitting from the improved performance and flexibility of recently introduced electron imaging detectors in which electrons are directly incident on backthinned CMOS sensors. There are currently three commercially available detectors of this type: the Direct Electron DE-20, the FEI Falcon II and the Gatan K2 Summit. These have different characteristics and so it is important to compare their imaging properties carefully with a view to optimise how each is used. Results at 300 keV for both the modulation transfer function (MTF) and the detective quantum efficiency (DQE) are presented. Of these, the DQE is the most important in the study of radiation sensitive samples where detector performance is crucial. We find that all three detectors have a better DQE than film. The K2 Summit has the best DQE at low spatial frequencies but with increasing spatial frequency its DQE falls below that of the Falcon II. Highlights • Three direct electron detectors offer better DQE than film at 300 keV. • Recorded 300 keV electron events on the detectors have very similar Landau distributions. • The Gatan K2 Summit detector has the highest DQE at low spatial frequency. • The FEI Falcon II detector has the highest DQE beyond one half the Nyquist frequency. • The Direct Electron DE-20 detector has the fastest data acquisition rate. |
| Databáze: | OpenAIRE |
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